Anti-VPS54 antibody [EPR28123-81] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-VPS54 antibody [EPR28123-81] - BSA and Azide free (AB307679)

This data was developed using ab307678, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Samples are non-boiled as boiling may cause protein aggregates.

All lanes:

Western blot - Anti-VPS54 antibody [EPR28123-81] (<a href='/en-us/products/primary-antibodies/vps54-antibody-epr28123-81-ab307678'>ab307678</a>) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-VPS54 antibody [EPR28123-81] - BSA and Azide free (AB307679)

This data was developed using ab307678, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 180 seconds

All lanes:

Western blot - Anti-VPS54 antibody [EPR28123-81] (<a href='/en-us/products/primary-antibodies/vps54-antibody-epr28123-81-ab307678'>ab307678</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeti VPS54 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 110 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-VPS54 antibody [EPR28123-81] - BSA and Azide free (AB307679)

This data was developed using ab307678, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Lane1 is non-boiled as boiling may cause protein aggregates.

All lanes:

Western blot - Anti-VPS54 antibody [EPR28123-81] (<a href='/en-us/products/primary-antibodies/vps54-antibody-epr28123-81-ab307678'>ab307678</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

true

Exposure time: 180s