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AB211358

Anti-VRK1 antibody

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(2 Publications)

Rabbit Polyclonal VRK1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human VRK1 aa 1-150.

View Alternative Names

Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody (AB211358)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody (AB211358)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized U-251 MG cells labeling VRK1 with ab211358 at 4 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRK1 antibody (AB211358)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VRK1 antibody (AB211358)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling VRK1 with ab211358 at 1/200 dilution.

Western blot - Anti-VRK1 antibody (AB211358)
  • WB

Supplier Data

Western blot - Anti-VRK1 antibody (AB211358)

All lanes:

Western blot - Anti-VRK1 antibody (ab211358) at 0.4 µg/mL

Lane 1:

Rh30 cell lysate with siRNA control

Lane 2:

Rh30 cell lysate with siRNA Target specific probe

Predicted band size: 45 kDa

false

Western blot - Anti-VRK1 antibody (AB211358)
  • WB

Supplier Data

Western blot - Anti-VRK1 antibody (AB211358)

All lanes:

Western blot - Anti-VRK1 antibody (ab211358) at 0.4 µg/mL

All lanes:

HL-60 (Human promyelocytic leukemia) cell lysate

Predicted band size: 45 kDa,73 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human VRK1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q99986

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Alternative Products

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed : 14645249, PubMed : 18617507, PubMed : 19103756, PubMed : 33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed : 31527692, PubMed : 37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed : 33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed : 21920476, PubMed : 31090908, PubMed : 31527692). Involved in Golgi disassembly during the cell cycle : following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed : 19103756). Phosphorylates BANF1 : disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed : 16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed : 10951572, PubMed : 31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed : 15105425). Phosphorylates JUN (PubMed : 31527692).
See full target information VRK1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:601055 PubMed33330099

2020

Long Noncoding RNA AFAP1-AS1 Is a Critical Regulator of Nasopharyngeal Carcinoma Tumorigenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Min Fang,Minjun Zhang,Yiqing Wang,Fangqiang Wei,Jianhui Wu,Xiaozhou Mou,Yigan Zhang,Xiaodong Liang,Jianming Tang

Oncology letters 20:1163-1170 PubMed32724356

2020

Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Ren,Jie Geng,Chao Xiong,Xuebing Li,Yuqing Li,Jin Li,Hongchun Liu
View all publications
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Product promise

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