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AB241103

Anti-VRK1 antibody

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(1 Publication)

Rabbit Polyclonal VRK1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human VRK1 aa 300 to C-terminus.

View Alternative Names

Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1

2 Images
Immunoprecipitation - Anti-VRK1 antibody (AB241103)
  • IP

Supplier Data

Immunoprecipitation - Anti-VRK1 antibody (AB241103)

VRK1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241103 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241103 at 1 μg/ml.

Lane 1 : ab241103 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-VRK1 antibody (ab241103)

Predicted band size: 45 kDa

false

Western blot - Anti-VRK1 antibody (AB241103)
  • WB

Supplier Data

Western blot - Anti-VRK1 antibody (AB241103)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-VRK1 antibody (ab241103) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Predicted band size: 45 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human VRK1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99986

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Antibody was affinity purified using an epitope specific to VRK1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed : 14645249, PubMed : 18617507, PubMed : 19103756, PubMed : 33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed : 31527692, PubMed : 37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed : 33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed : 21920476, PubMed : 31090908, PubMed : 31527692). Involved in Golgi disassembly during the cell cycle : following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed : 19103756). Phosphorylates BANF1 : disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed : 16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed : 10951572, PubMed : 31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed : 15105425). Phosphorylates JUN (PubMed : 31527692).
See full target information VRK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ vaccines 7:24 PubMed35197485

2022

An adjuvanted subunit SARS-CoV-2 spike protein vaccine provides protection against Covid-19 infection and transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Kairat Tabynov,Nurkeldi Turebekov,Meruert Babayeva,Gleb Fomin,Toktassyn Yerubayev,Tlektes Yespolov,Lei Li,Gourapura J Renukaradhya,Nikolai Petrovsky,Kaissar Tabynov
View all publications
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