Anti-VSIG4 antibody [EPR22576-125]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] (AB252932)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] (AB252932)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-125] (AB252932)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252932 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

• WB

Unknown

Western blot - Anti-VSIG4 antibody [EPR22576-125] (AB252932)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :

Lane 1 : 125 seconds

Lane 2 : 3 minutes

Lanes 3-4 : 125 seconds

The molecular weight observed is consistent with what has been described in previous literatures (PMID : 16009265, 17016562)

The expression profile observed is consistent with the literatures (PMID : 16009265, 17016562)

Negative control : Jurkat (PMID : 16009265)

Lanes 1, 3 and 4 were developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-VSIG4 antibody [EPR22576-125] (ab252932) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate at 20 µg

Lane 4:

THP-1 (human monocytic leukemia cell line), whole cell lysate at 20 µg

Secondary

Lane 1:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 2 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 37 kDa,50 kDa

false

• WB

Unknown

Western blot - Anti-VSIG4 antibody [EPR22576-125] (AB252932)

Blocking/diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-VSIG4 antibody [EPR22576-125] (ab252932) at 1/1000 dilution

All lanes:

His-tagged human VSIG4 recombinant protein (aa 19-232) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

Observed band size: 27 kDa

false