Anti-VSIG4 antibody [EPR22576-70]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

IHC image of VSIG4 staining in a section of frozen normal mouse liver performed on a Leica Biosystems BOND® RX instrument using the standard protocol for frozen sections. The section was fixed using 10% paraformaldehyde for 10 mins at room temperature prior to staining. The section was incubated with ab252933, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times."

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of mouse liver (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

• WB

Unknown

Western blot - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 125 seconds.

The molecular weight observed is consistent with what has been described in previous literatures (PMID : 16009265, 17016562)

The expression profile observed is consistent with the literatures (PMID : 16009265, 17016562).

Negative control : Jurkat (PMID : 16009265).

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-VSIG4 antibody [EPR22576-70] (ab252933) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood), whole cell lysate at 20 µg

Lane 4:

THP-1 (human monocytic leukemia cell line), whole cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Lanes 3 - 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 37 kDa,50 kDa

false

• WB

Unknown

Western blot - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID : 17016562, PMID : 17016562).

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-VSIG4 antibody [EPR22576-70] (ab252933) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 37 kDa

false

• WB

Unknown

Western blot - Anti-VSIG4 antibody [EPR22576-70] (AB252933)

Blocking /diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-VSIG4 antibody [EPR22576-70] (ab252933) at 1/1000 dilution

All lanes:

His-tagged human VSIG4 recombinant protein (aa 19-232) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 44 kDa

Observed band size: 27 kDa

false