AB255692

Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal VSIG4 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

CRIg, Z39IG, UNQ317/PRO362, VSIG4, V-set and immunoglobulin domain-containing protein 4, Protein Z39Ig

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stroma of human placenta (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on macrophages of human lung (PMID : 17016562) is observed. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of human liver (PMID : 17016562) is observed. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using ab208572 retrieval buffer.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VSIG4 with ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on Kupffer cells of mouse liver (PMID : 17016562) is observed. Counterstained with Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252933).

Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

This data was developed using ab252933, the same antibody clone in a different buffer formulation.

IHC image of VSIG4 staining in a section of frozen normal mouse liver performed on a Leica Biosystems BOND® RX instrument using the standard protocol for frozen sections. The section was fixed using 10% paraformaldehyde for 10 mins at room temperature prior to staining. The section was incubated with ab252933, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times."

Unconjugated

Anti-VSIG4 antibody [EPR22576-70]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22576-70

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Perform heat mediated antigen retrieval using <a href='/en-us/products/ihc-kits/universal-hier-antigen-retrieval-reagent-10x-ab208572'>ab208572</a> retrieval buffer.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

ab255692 is the carrier-free version of ab252933.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.

See full target information VSIG4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:86 PubMed40307956

2025

Prion replication in organotypic brain slice cultures is distinct from in vivo inoculation and is species dependent.

Applications

Unspecified application

Species

Unspecified reactive species

Jessy A Slota,Lise Lamoureux,Jennifer Myskiw,Kathy L Frost,Sarah J Medina,Dominic M S Kielich,Melanie Leonhardt,Gunjan Thapar,Ben A Bailey-Elkin,Stephanie A Booth

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (1)

Product promise