Rabbit Recombinant Monoclonal VSIG4 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106540.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113648.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13866.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120577.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)")
![Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-frozen-sections-img444818.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (AB255692)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Expected | Tested | Not recommended | Expected |
| Mouse | Not recommended | Not recommended | Expected | Expected | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 retrieval buffer. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
Alternative names
CRIg, Z39IG, UNQ317/PRO362, VSIG4, V-set and immunoglobulin domain-containing protein 4, Protein Z39Ig
Recommended products
Rabbit Recombinant Monoclonal VSIG4 antibody. Carrier free. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22576-70
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255692 is the carrier-free version of Anti-VSIG4 antibody [EPR22576-70] ab252933.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106540.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling VSIG4 with Anti-VSIG4 antibody [EPR22576-70] ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Kupffer cells of human liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 retrieval buffer.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VSIG4 antibody [EPR22576-70] ab252933).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img113648.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling VSIG4 with Anti-VSIG4 antibody [EPR22576-70] ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on macrophages of human lung (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VSIG4 antibody [EPR22576-70] ab252933).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img13866.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling VSIG4 with Anti-VSIG4 antibody [EPR22576-70] ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stroma of human placenta (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VSIG4 antibody [EPR22576-70] ab252933).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120577.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling VSIG4 with Anti-VSIG4 antibody [EPR22576-70] ab252933 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on Kupffer cells of mouse liver (PMID: 17016562) is observed. Counterstained with Hematoxylin.
Heat mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 retrieval buffer.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-VSIG4 antibody [EPR22576-70] ab252933).
![Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692), expandable thumbnail](https://content.abcam.com/products/images/vsig4-antibody-epr22576-70-bsa-and-azide-free-ab255692--immunohistochemistry-frozen-sections-img444818.jpg "Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)")
Immunohistochemistry (Frozen sections) - Anti-VSIG4 antibody [EPR22576-70] - BSA and Azide free (ab255692)
This data was developed using Anti-VSIG4 antibody [EPR22576-70] ab252933, the same antibody clone in a different buffer formulation.
IHC image of VSIG4 staining in a section of frozen normal mouse liver performed on a Leica Biosystems BOND® RX instrument using the standard protocol for frozen sections. The section was fixed using 10% paraformaldehyde for 10 mins at room temperature prior to staining. The section was incubated with Anti-VSIG4 antibody [EPR22576-70] ab252933, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times."
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