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AB1874

Anti-VSV-G tag antibody

3

(2 Reviews)

|

(22 Publications)

Rabbit Polyclonal GLYCO antibody. Suitable for ICC, IP, WB, ICC/IF and reacts with Tag samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Vesicular stomatitis Indiana virus (strain 98COE North America) G aa 500 to C-terminus.

View Alternative Names

Glycoprotein, G

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

IP, ICC, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Vesicular stomatitis Indiana virus (strain 98COE North America) G aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8B0I1

Specificity

This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This affinity purified antibody is directed against VSV-G protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Attaches the virus to host cellular receptor, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane. In neurons, neo-synthesized glycoproteins are sorted to the dendrites, where the virus buds (By similarity).
See full target information G

Additional targets

G,,,G

Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 97:e0078623 PubMed37796126

2023

Ubiquitin E3 ligase SPOP is a host negative regulator of enterovirus 71-encoded 2A protease.

Applications

Unspecified application

Species

Unspecified reactive species

Lichao Zang,Xinyu Yang,Yan Chen,Fan Huang,Yukang Yuan,Xiangjie Chen,Yibo Zuo,Ying Miao,Jin Gu,Hui Guo,Wenxin Xia,Yang Peng,Mengyuan Tang,Ziwei Huang,Yangyang Wang,Jinhong Ma,Jingting Jiang,Wei Zhou,Hui Zheng,Weifeng Shi

Immunology 170:527-539 PubMed37641430

2023

Ceftazidime reduces cellular Skp2 to promote type-I interferon activity.

Applications

Unspecified application

Species

Unspecified reactive species

Caixia Qiao,Fan Huang,Jiuyi He,Qiuyu Wu,Zhijin Zheng,Tingting Zhang,Ying Miao,Yukang Yuan,Xiangjie Chen,Qian Du,Yang Xu,Depei Wu,Zhengyuan Yu,Hui Zheng

Nature structural & molecular biology 30:370-382 PubMed36624347

2023

HIV-1 is dependent on its immature lattice to recruit IP6 for mature capsid assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Nadine Renner,Alex Kleinpeter,Donna L Mallery,Anna Albecka,K M Rifat Faysal,Till Böcking,Adolfo Saiardi,Eric O Freed,Leo C James

Science advances 8:eabj3887 PubMed35394840

2022

LATS1 is a central signal transmitter for achieving full type-I interferon activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yibo Zuo,Jiuyi He,Siying Liu,Ying Xu,Jin Liu,Caixia Qiao,Lichao Zang,Wenhuan Sun,Yukang Yuan,Hongguang Zhang,Xiangjie Chen,Lincong Jin,Ying Miao,Fan Huang,Tengfei Ren,Jun Wang,Feng Qian,Chuanwu Zhu,Wei Zhang,Yaobo Liu,Guoqiang Xu,Feng Ma,Hui Zheng

PLoS pathogens 17:e1009530 PubMed33909701

2021

The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NFκB transcripts to dampen antiviral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xu,Yunhong Cai,ZhenBang Ma,Bo Jiang,Wenxiao Liu,Jing Cheng,Nannan Guo,Zishu Wang,Joshua E Sealy,Cuiping Song,Xiaojia Wang,Yongqing Li

Immunology 163:278-292 PubMed33513265

2021

Ubiquitin E3 ligase MID1 inhibits the innate immune response by ubiquitinating IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangjie Chen,Ying Xu,Wenhui Tu,Fan Huang,Yibo Zuo,Hong-Guang Zhang,Lincong Jin,Qian Feng,Tengfei Ren,Jiuyi He,Ying Miao,Yukang Yuan,Qian Zhao,Jiapeng Liu,Renxia Zhang,Li Zhu,Feng Qian,Chuanwu Zhu,Hui Zheng,Jun Wang

Cell reports 33:108490 PubMed33296647

2020

Nuclear cGAS Functions Non-canonically to Enhance Antiviral Immunity via Recruiting Methyltransferase Prmt5.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Cui,Qiuya Yu,Lei Chu,Ye Cui,Ming Ding,Quanyi Wang,Hongyun Wang,Yu Chen,Xing Liu,Chen Wang

Journal of neurochemistry 157:1284-1299 PubMed33180957

2020

Ubiquitination and the proteasome rather than caspase-3-mediated C-terminal cleavage are involved in the EAAT2 degradation by staurosporine-induced cellular stress.

Applications

Unspecified application

Species

Unspecified reactive species

Timo-Daniel Voss,Maria Gerget,Birgit Linkus,Bjoern von Einem,G Bernhard Landwehrmeyer,Jan Lewerenz

Cell stem cell 28:63-78.e7 PubMed33053374

2020

Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/β-Catenin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Li,Maorong Chen,Jiliang Hu,Ren Sheng,Qirong Lin,Xi He,Ming Guo

Cell discovery 4:65 PubMed30603101

2018

A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Xiang Ni,Nan Zhou,Wen-Qian Xue,Li Rong,Wing-Ho Yung,Rao-Zhou Lin,Richard Yi-Tsun Kao,Zhi-Gang Duan,Hai-Tao Sun,Hua-Rui Gong,Xu-Ming Tang,Meng-Fei Liu,Wen Zhang,Shuang Qi,Sookja Chung,You-Qiang Song,Jian-Dong Huang
View all publications

Product promise

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