Anti-VSV-G tag antibody [EPR28362-204]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK293T (human embryonic kidney epithelial cell) co-transfected with a VSVG-tag expression vector containing a his tag. cells labeling VSV-G tag with ab309106 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Immunohistochemical analysis of paraffin-embedded cell pellets A) HEK293T (human embryonic kidney epithelial cell) co-transfected with a VSVG-tag expression vector containing a his tag and (B) HEK-293T cells transfected with empty vector staining VSV-G tag with ab309106 at 1/5000 dilution (0.101 ug/ml) followed by ready to use LeicaDS9800 (Bond^®Polymer Refine Detection).

Cytoplasmic staining on VSVG-tag overexpression HEK293T cells, no staining on parental HEK293T cells.
The section was incubated with ab309106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue staining VSV-G tag with ab309106 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human skeletal muscle. The section was incubated with ab309106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labeling VSV-G tag with ab309106 at 1/2000 (0.252 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with a mouse VSV-G-Lgr5 expression vector containing a myc tag. The image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Myc-Tag Mouse mab (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue staining VSV-G tag with ab309106 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse skeletal muscle. The section was incubated with ab309106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue staining VSV-G tag with ab309106 at 1/5000 (0.101 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat skeletal muscle. The section was incubated with ab309106 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

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Western blot - Anti-VSV-G tag antibody [EPR28362-204] (AB309106)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-VSV-G tag antibody [EPR28362-204] (ab309106) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector (vector control), whole cell lysate at 10 µg

Lane 2:

293T cells transfected with VSV-G-Lgr5 (mouse) expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T cells transfected with mouse Lgr5 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 100 kDa

false

Exposure time: 8s