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AB50549

Anti-VSV-G tag antibody [P5D4]

4

(1 Review)

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(27 Publications)

Anti-VSV-G tag antibody [P5D4] (ab50549) is a mouse monoclonal antibody detecting VSV-G tag in Western Blot, IP.

- Over 20 publications
- Trusted since 2007

View Alternative Names

Glycoprotein, G

2 Images
Immunocytochemistry - Anti-VSV-G tag antibody [P5D4] (AB50549)
  • ICC

Supplier Data

Immunocytochemistry - Anti-VSV-G tag antibody [P5D4] (AB50549)

COS7 cells over-expressing VSV-G tagged vinculin were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 5 μg/ml ab50549. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Western blot - Anti-VSV-G tag antibody [P5D4] (AB50549)
  • WB

Supplier Data

Western blot - Anti-VSV-G tag antibody [P5D4] (AB50549)

Lane 1:

Western blot - Anti-VSV-G tag antibody [P5D4] (ab50549) at 0.5 µg/mL

Lane 2:

Negative control: without first antibody

Lane 1:

Whole extract of HEK-293T cells over-expressing VSV-G-tagged Vinculin

Lane 2:

Whole extract of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells over-expressing VSV-G-tagged Vinculin

Secondary

All lanes:

Goat Anti-Mouse IgG-Peroxidase

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P5D4

Isotype

IgG1

Carrier free

No

Applications

ICC, IP, WB

applications

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Reactivity data

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Product details

What is this antibody validated in?
Anti-VSV-G tag antibody [P5D4] (ab50549) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP). Tagged protein detection
VSV-G tag antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Trusted by the scientific community
Anti-VSV-G tag [P5D4] (ab50549) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
Purified from Tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Attaches the virus to host cellular receptor, inducing clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane. In neurons, neo-synthesized glycoproteins are sorted to the dendrites, where the virus buds.
See full target information G
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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111384 PubMed39669425

2024

Phosphorylation of Optineurin by protein kinase D regulates Parkin-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Weil,Emmanuel Laplantine,Messaouda Attailia,Anne Oudin,Shannel Curic,Aya Yokota,Elie Banide,Pierre Génin

EMBO reports 25:5719-5742 PubMed39511422

2024

In situ cell-surface conformation of the TCR-CD3 signaling complex.

Applications

Unspecified application

Species

Unspecified reactive species

Aswin Natarajan,Yogambigai Velmurugu,Manuel Becerra Flores,Fatoumatta Dibba,Saikiran Beesam,Sally Kikvadze,Xiaotian Wang,Wenjuan Wang,Tianqi Li,Hye Won Shin,Timothy Cardozo,Michelle Krogsgaard

Journal of virology 98:e0001624 PubMed38563732

2024

The Ca-dependent phosphatase calcineurin dephosphorylates TBK1 to suppress antiviral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Qu,Siyuan Wang,Hui Jiang,Qingyi Wang,Ying Liao,Xusheng Qiu,Lei Tan,Cuiping Song,Chan Ding,Yingjie Sun,Zengqi Yang

Nature biomedical engineering 7:1571-1582 PubMed37142844

2023

Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Phillips,Adam D Silverman,Aric Joneja,Michael Liu,Carl Brown,Paul Carlson,Christine Coticchia,Kristen Shytle,Alex Larsen,Nadish Goyal,Vincent Cai,Jason Huang,Jennifer E Hickey,Emily Ryan,Joycelynn Acheampong,Pradeep Ramesh,James J Collins,William J Blake

Cell reports 41:111738 PubMed36450259

2022

Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchun Wang,Yuzhen Wang,Lu Ding,Xiaolei Ren,Bo Wang,Liyuan Wang,Songbo Zhao,Xuetian Yue,Zhuanchang Wu,Chunyang Li,Xiaohong Liang,Chunhong Ma,Lifen Gao

International journal of molecular sciences 23: PubMed35408951

2022

Comparison of the Cisterna Maturation-Progression Model with the Kiss-and-Run Model of Intra-Golgi Transport: Role of Cisternal Pores and Cargo Domains.

Applications

Unspecified application

Species

Unspecified reactive species

Galina V Beznoussenko,Hee-Seok Kweon,Irina S Sesorova,Alexander A Mironov

Cell reports 38:110396 PubMed35172151

2022

FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Viacheslav Malikov,Nathan Meade,Lacy M Simons,Judd F Hultquist,Mojgan H Naghavi

mBio 12:e0100521 PubMed34125604

2021

Caspase-Dependent Cleavage of DDX21 Suppresses Host Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wu,Yang Qu,Shengqing Yu,Sa Wang,Yuncong Yin,Qinfang Liu,Chunchun Meng,Ying Liao,Zaib Ur Rehman,Lei Tan,Cuiping Song,Xusheng Qiu,Weiwei Liu,Chan Ding,Yingjie Sun

Experimental cell research 396:112332 PubMed33065113

2020

RNA helicase DDX5 suppresses IFN-I antiviral innate immune response by interacting with PP2A-Cβ to deactivate IRF3.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zan,Ruixian Xu,Xialin Tang,Minyi Lu,Shanshan Xie,Jun Cai,Zhi Huang,Jinyang Zhang

Frontiers in bioengineering and biotechnology 8:574007 PubMed33042975

2020

Extracellular Vesicles-Mimetic Encapsulation Improves Oncolytic Viro-Immunotherapy in Tumors With Low Coxsackie and Adenovirus Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghui Zhang,Junyi Wu,Hailin Zhang,Jiwu Wei,Junhua Wu
View all publications
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Product promise

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