Rabbit Polyclonal VT/Stx antibody. Suitable for ELISA, WB, IP and reacts with Escherichia coli, Purified native protein samples.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.09% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| ELISA | WB | IP | |
|---|---|---|---|
| Escherichia coli | Tested | Tested | Tested |
| Purified native protein | Not recommended | Tested | Not recommended |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info 1/2000 | Notes - |
Species Escherichia coli | Dilution info 1/2000 | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Escherichia coli | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info - | Notes - |
Target data
Recommended products
Rabbit Polyclonal VT/Stx antibody. Suitable for ELISA, WB, IP and reacts with Escherichia coli, Purified native protein samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.09% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Purity
- Whole antiserum
- Specificity
VT1 and VT2 of E. coli VTEC strain and Shiga toxin of Shigella dysenteriae 1.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunoprecipitation - Anti-VT/Stx antibody (ab188542)
Immunoprecipitation of VT1 from culture medium of VTEC with ab188542. Arrows shows subunit A and subunit B of VT1. Heavy chain and Light chain indicate those of IgG.
All lanes: Immunoprecipitation - Anti-VT/Stx antibody (ab188542)
Western blot - Anti-VT/Stx antibody (ab188542)
All lanes: Western blot - Anti-VT/Stx antibody (ab188542) at 1/2000 dilution
Lane 1: culture medium of VTEC
Lane 2: crude extracts of VTEC cells
Predicted band size: 36 kDa
Western blot - Anti-VT/Stx antibody (ab188542)
All lanes: Western blot - Anti-VT/Stx antibody (ab188542)
Lane 1: Culture medium of E. coli O157:H7
Lane 2: Purified VT1
Lane 3: Purified VT2
Predicted band size: 36 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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