Rabbit Recombinant Monoclonal VTI1B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-VTI1B antibody [EPR15506(B)] (AB184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--western-blot-img154097.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VTI1B antibody [EPR15506(B)] (AB184170)")
![Flow Cytometry (Intracellular) - Anti-VTI1B antibody [EPR15506(B)] (AB184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--flow-cytometry-intracellular-img79593.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-VTI1B antibody [EPR15506(B)] (AB184170)")
![Western blot - Anti-VTI1B antibody [EPR15506(B)] (AB184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--western-blot-img104945.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VTI1B antibody [EPR15506(B)] (AB184170)")
![Western blot - Anti-VTI1B antibody [EPR15506(B)] (AB184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--western-blot-img79817.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-VTI1B antibody [EPR15506(B)] (AB184170)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (AB184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120456.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (AB184170)")
Publications
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- The Journal of cell biology 221:2022VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Li,Fengping Feng,Han Feng,Pengkai Hu,Yanhong Xue,Tao Xu,Eli SongPubMed 36053215
- eLife 10:2021An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert Coukos,David Yao,Mateo I Sanchez,Eric T Strand,Meagan E Olive,Namrata D Udeshi,Jonathan S Weissman,Steven A Carr,Michael C Bassik,Alice Y TingPubMed 34414886
- Journal of cell science 133:2020Invariant chain regulates endosomal fusion and maturation through an interaction with the SNARE Vti1b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAzzurra Margiotta et. al.PubMed 32907852
- PloS one 14:e02141102019The Gag protein PEG10 binds to RNA and regulates trophoblast stem cell lineage specification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMona Abed et. al.PubMed 30951545
- Immunologic research 65:1031-10452017Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin.Applications:Flow CytReactive species:HumanKelsey Lesteberg,Jordan Orange,George MakedonasPubMed 28822075
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/170 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. May be concerned with increased secretion of cytokines associated with cellular senescence.
Alternative names
VTI1, VTI1L, VTI1L1, VTI2, VTI1B, Vesicle transport through interaction with t-SNAREs homolog 1B, Vesicle transport v-SNARE protein Vti1-like 1, Vti1-rp1
Recommended products
Rabbit Recombinant Monoclonal VTI1B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15506(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--western-blot-img154097.jpg "Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
Lanes 1-3: Merged signal (red and green). Green - ab184170 observed at 28 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab184170 Anti-VTI1B antibody [EPR15506(B)] was shown to specifically react with VTI1B in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human VTI1B knockout HeLa cell line ab265483 (knockout cell lysate Human VTI1B knockout HeLa cell lysate ab257797) was used. Wild-type and VTI1B knockout samples were subjected to SDS-PAGE. ab184170 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: VTI1B knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human VTI1B knockout HeLa cell line (Human VTI1B knockout HeLa cell line ab265483)
Lane 3: MCF7 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 28 kDa
![Flow Cytometry (Intracellular) - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--flow-cytometry-intracellular-img79593.jpg "Flow Cytometry (Intracellular) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
Flow Cytometry (Intracellular) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
Intracellular flow cytometric analysis of Hela cells (2% paraformaldehyde-fixed) labeling VTI1B with ab184170 at 1/170 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--western-blot-img104945.jpg "Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
All lanes: Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/2000 dilution
Lane 1: MCF7 cell lysate at 20 µg
Lane 2: HeLa cell lysate at 20 µg
Lane 3: Human fetal liver lysate at 20 µg
Lane 4: Human fetal lung lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
![Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--western-blot-img79817.jpg "Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
All lanes: Western blot - Anti-VTI1B antibody [EPR15506(B)] (ab184170) at 1/10000 dilution
All lanes: MCF7 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120456.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma labeling VTI1B with ab184170 at 1:100 diluton and HRP polymer. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--immunocytochemistry-immunofluorescence-img58502.jpg "Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling VTI1B with ab184170 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170), expandable thumbnail](https://content.abcam.com/products/images/vti1b-antibody-epr15506b-ab184170--immunocytochemistry-immunofluorescence-img58502.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-VTI1B antibody [EPR15506(B)] (ab184170)")
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