Anti-VZV gE protein antibody [#9]

• WB

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Western blot - Anti-VZV gE protein antibody [#9] (AB272686)

The broad band in WB reflects multiple species of gE which are glycosylated at different levels.

All lanes:

Western blot - Anti-VZV gE protein antibody [#9] (ab272686) at 1/5000 dilution

Lane 1:

VZV strain pOka infected MRC-5 (human fibroblasts lung cell line) whole cell lysate

Lane 2:

MRC-5 whole cell lysate

Predicted band size: 70 kDa

false

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-VZV gE protein antibody [#9] (AB272686)

Immunofluorescence analysis of VZV-infected MRC-5 (human fibroblasts lung cell line) cells labeling VZV gE protein at 1/100 dilution, followed by an Alexa-Fluor^®488 donkey anti-mouse IgG [H+L] at 1/200 dilution (green). Nuclei were stained with (blue).

• ELISA

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ELISA - Anti-VZV gE protein antibody [#9] (AB272686)

Indicated amounts of VZV-lysate was coated per well of the ELISA plate. After blocking with 5% skim milk, 100 µl of ab272686 at the 1/5000 dilution was added to the each well. HRP-conjugate goat antimouse IgG (100μl, 1/4000 dilution) was added. OPD (ortho-phenylenediamine) was used as substrate. Optical densities (OD) measured at 450nm. Dynamic range was 200 pg to 10ng under these conditions.

• ELISA

Supplier Data

ELISA - Anti-VZV gE protein antibody [#9] (AB272686)

VZV-lysate (100 µl, 1 μg/ml) was coated onto the wells of the ELISA plate. After blocking with 5% skim milk, 100 µl of ab272686 at the indicated dilution was added to the each well. Goat anti-mouse IgG-HRP (100μl, 1/4000 dilution) was added. OPD (ortho-phenylenediamine) was used as a substrate. Optical densities (OD) measured at 450nm.