AB109537

Anti-WAPL/FOE antibody [EPR3407(2)]

Name: Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) | Abcam
Brand: Abcam Limited
SKU: AB109537
Price: 530 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal WAPL/FOE antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

FOE, KIAA0261, WAPAL, WAPL, Wings apart-like protein homolog, Friend of EBNA2 protein, WAPL cohesin release factor

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

ab109537 at 1/100 dilution staining WAPL/FOE in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunofluorescence.

AbReview32141****

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

All lanes:

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/10000 dilution

Lane 1:

Human U2OS cells treated with siRNA control. at 90 µg

Lane 2:

Human U2OS cells treated with siRNA #1 against WAPL/FOE at 90 µg

Lane 3:

Human U2OS cells treated with siRNA #2 against WAPL/FOE at 90 µg

Secondary

All lanes:

Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution

Predicted band size: 133 kDa

Observed band size: 120 kDa,130 kDa,145 kDa,150 kDa

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Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

All lanes:

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (ab109537) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 133 kDa,74 kDa

Observed band size: 70 kDa

false

421 Alexa Fluor® 405

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed
603 Alexa Fluor® 568

Goat Anti-Rabbit IgG Fc (Alexa Fluor® 568) preadsorbed

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3407(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin (PubMed : 26299517). Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

See full target information WAPL

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 34:1619-1636 PubMed33122293

2020

Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Pinzaru,Mike Kareh,Noa Lamm,Eros Lazzerini-Denchi,Anthony J Cesare,Agnel Sfeir

The EMBO journal 35:2686-2698 PubMed27872142

2016

Cohesin acetylation and Wapl-Pds5 oppositely regulate translocation of cohesin along DNA.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Kanke,Eri Tahara,Pim J Huis In't Veld,Tomoko Nishiyama

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WAPL/FOE antibody [EPR3407(2)]

Immunocytochemistry/ Immunofluorescence - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

Western blot - Anti-WAPL/FOE antibody [EPR3407(2)] (AB109537)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (2)

Product promise