Rabbit Polyclonal WASP/Wiskott-Aldrich syndrome protein antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WAS aa 1-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WAS aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P42768
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905). Important for efficient actin polymerization (PubMed:12235133, PubMed:16275905, PubMed:8625410). Possible regulator of lymphocyte and platelet function (PubMed:9405671). Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed:18650809). In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:20574068). Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
IMD2, WAS, Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp
Recommended products
Rabbit Polyclonal WASP/Wiskott-Aldrich syndrome protein antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WAS aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WAS aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P42768
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatograph.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216)
Formalin-fixed, paraffin-embedded human spleen tissue stained for WASP/Wiskott-Aldrich syndrome protein using ab233216 at 20 μg/mL in immunohistochemical analysis. DAB staining.
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216)
All lanes: Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216) at 1 µg/mL
All lanes: HL-60 (human promyelocytic leukemia cell line) cell lysate
SecondaryAll lanes: HRP-Linked Goat Anti-Rabbit IgG Polyclonal at 0.2 µg/mL
Predicted band size: 53 kDa
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216)
All lanes: Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab233216) at 2 µg/mL
All lanes: Recombinant human WASP/Wiskott-Aldrich syndrome protein
Predicted band size: 53 kDa
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