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AB264134

Anti-WASP/Wiskott-Aldrich syndrome protein antibody

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Rabbit Polyclonal WASP/Wiskott-Aldrich syndrome protein antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human WAS aa 400-500.

View Alternative Names

IMD2, WAS, Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp

2 Images
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (AB264134)
  • WB

Supplier Data

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (AB264134)

4-20% SDS-PAGE

All lanes:

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab264134) at 0.4 µg/mL

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 5 µg

Predicted band size: 53 kDa

false

Exposure time: 30s

Immunoprecipitation - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (AB264134)
  • IP

Supplier Data

Immunoprecipitation - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (AB264134)

WASP/Wiskott-Aldrich syndrome protein was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab264134 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264134 at 1 μg/ml.

Lane 1 : ab264134 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

All lanes:

Immunoprecipitation - Anti-WASP/Wiskott-Aldrich syndrome protein antibody (ab264134)

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human WAS aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

P42768

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed : 12235133, PubMed : 12769847, PubMed : 16275905). Important for efficient actin polymerization (PubMed : 12235133, PubMed : 16275905, PubMed : 8625410). Possible regulator of lymphocyte and platelet function (PubMed : 9405671). Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed : 18650809). In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 20574068). Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information WAS

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