Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)

Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)

Immunocytochemistry analysis of TF-1 (Human bone marrow erythroblast) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1/50 dilution (5.88 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Unknown

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830) at 1/10000 dilution

Lane 1:

TF-1 (Human Erythroleukemia erythroblast) whole cell lysate at 15 µg

Lane 2:

U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 60 kDa

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