Rabbit Recombinant Monoclonal WASP/Wiskott-Aldrich syndrome protein antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Images
![Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830), expandable thumbnail](https://content.abcam.com/products/images/wasp-wiskott-aldrich-syndrome-protein-antibody-ep2541y-ab75830--western-blot-img7645.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)")
![Immunocytochemistry/ Immunofluorescence - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830), expandable thumbnail](https://content.abcam.com/products/images/wasp-wiskott-aldrich-syndrome-protein-antibody-ep2541y-ab75830--immunocytochemistry-immunofluorescence-img70717.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)")
![Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830), expandable thumbnail](https://content.abcam.com/products/images/wasp-wiskott-aldrich-syndrome-protein-antibody-ep2541y-ab75830--flow-cytometry-intracellular-img37528.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (AB75830)")
Publications
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- Nature medicine 28:71-802022Long-term safety and efficacy of lentiviral hematopoietic stem/progenitor cell gene therapy for Wiskott-Aldrich syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Magnani,M Semeraro,F Adam,C Booth,L Dupré,E C Morris,A Gabrion,C Roudaut,D Borgel,A Toubert,E Clave,C Abdo,G Gorochov,R Petermann,M Guiot,M Miyara,D Moshous,E Magrin,A Denis,F Suarez,C Lagresle,A M Roche,J Everett,A Trinquand,M Guisset,J Xu Bayford,S Hacein-Bey-Abina,A Kauskot,R Elfeky,C Rivat,S Abbas,H B Gaspar,E Macintyre,C Picard,F D Bushman,A Galy,A Fischer,E Six,A J Thrasher,M CavazzanaPubMed 35075289
- Scandinavian journal of immunology 95:e131152021Clinical and molecular characteristics of Wiskott-Aldrich Syndrome in five unrelated Chinese families.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiali Jiang,Junli Zhou,Manlv Wei,Sanjeev Singh,Lauriane Nikuze,Lifang Huang,Yuping Li,Jinxia Jiang,Hongying WeiPubMed 34758123
- Medicine 100:e255272021Clinical and genetic analysis of 2 rare cases of Wiskott-Aldrich syndrome from Chinese minorities: Two case reports.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaifeng Liu,Yanchun Wang,Yangfang Li,Lvyan Tao,Yu Zhang,Xiaoli He,Yuantao Zhou,Xiaoning Liu,Yan Wang,Li LiPubMed 33879693
- Nature communications 11:40342020Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott - Aldrich Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajeev Rai,Marianna Romito,Elizabeth Rivers,Giandomenico Turchiano,Georges Blattner,Winston Vetharoy,Dariusz Ladon,Geoffroy Andrieux,Fang Zhang,Marta Zinicola,Diego Leon-Rico,Giorgia Santilli,Adrian J Thrasher,Alessia CavazzaPubMed 32788576
- Journal of cell science 133:2020Myo1e modulates the recruitment of activated B cells to inguinal lymph nodes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel A Girón-Pérez et. al.PubMed 31964710
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905). Important for efficient actin polymerization (PubMed:12235133, PubMed:16275905, PubMed:8625410). Possible regulator of lymphocyte and platelet function (PubMed:9405671). Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed:18650809). In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:20574068). Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
Alternative names
IMD2, WAS, Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp
Recommended products
Rabbit Recombinant Monoclonal WASP/Wiskott-Aldrich syndrome protein antibody. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP2541Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830), expandable thumbnail](https://content.abcam.com/products/images/wasp-wiskott-aldrich-syndrome-protein-antibody-ep2541y-ab75830--western-blot-img7645.jpg "Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)")
Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830) at 1/10000 dilution
Lane 1: TF-1 (Human Erythroleukemia erythroblast) whole cell lysate at 15 µg
Lane 2: U-937 (Human histiocytic lymphoma monocyte) whole cell lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 60 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830), expandable thumbnail](https://content.abcam.com/products/images/wasp-wiskott-aldrich-syndrome-protein-antibody-ep2541y-ab75830--immunocytochemistry-immunofluorescence-img70717.jpg "Immunocytochemistry/ Immunofluorescence - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)")
Immunocytochemistry/ Immunofluorescence - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
Immunocytochemistry analysis of TF-1 (Human bone marrow erythroblast) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1/50 dilution (5.88 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488,Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830), expandable thumbnail](https://content.abcam.com/products/images/wasp-wiskott-aldrich-syndrome-protein-antibody-ep2541y-ab75830--flow-cytometry-intracellular-img37528.jpg "Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)")
Flow Cytometry (Intracellular) - Anti-WASP/Wiskott-Aldrich syndrome protein antibody [EP2541Y] (ab75830)
Intracellular Flow Cytometry analysis of U-937 (Human histiocytic lymphoma monocyte) cells labeling WASP/Wiskott-Aldrich syndrome protein with Purified ab75830 at 1/30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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