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AB59278

Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody

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(8 Publications)

Rabbit Polyclonal WASP/Wiskott-Aldrich syndrome protein phospho Y290 antibody. Suitable for ELISA, WB, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human WAS phospho Y290 aa 250-350.

View Alternative Names

IMD2, WAS, Actin nucleation-promoting factor WAS, Wiskott-Aldrich syndrome protein, WASp

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (AB59278)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (AB59278)

Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59278 at a dilution of 1/50.
Left image : no immunising peptide.
Right hand image : with immunising peptide.

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (AB59278)
  • WB

Unknown

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (AB59278)

All lanes:

Western blot - Anti-WASP/Wiskott-Aldrich syndrome protein (phospho Y290) antibody (ab59278) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells with no immunising peptide

Lane 2:

Extracts from HepG2 cells with immunising peptide

Predicted band size: 53 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human WAS phospho Y290 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P42768

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed : 12235133, PubMed : 12769847, PubMed : 16275905). Important for efficient actin polymerization (PubMed : 12235133, PubMed : 16275905, PubMed : 8625410). Possible regulator of lymphocyte and platelet function (PubMed : 9405671). Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria (PubMed : 18650809). In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed : 20574068). Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed : 29925947).
See full target information WAS phospho Y290
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40985892

2025

HMGB1 couples LEF1 to regulate B cell immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyue Chen,Ziyin Zhang,Nanshu Xiang,Li Luo,Xin Dai,Danqing Kang,Lu Yang,Yingzi Zhu,Jiang Chang,Yukai Jing,Na Li,Qianglin Chen,Panpan Jiang,Ju Liu,Yanmei Huang,Heather Miller,Xinyuan Zhou,Fang Zheng,Quan Gong,Chaohong Liu

Cell death & disease 16:433 PubMed40467564

2025

MBD2 promotes B cell differentiation and BCR signaling in systemic lupus erythematosus by regulating the LEF-1-PTEN-PI3K axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yukai Jing,Yunfei Zhang,Xiaocui Wang,Yufen Wang,Ying Hu,Bin Wen,Xin Dai,Xuemei Duan,Haonan Li,Shumin Dong,Ze Yan,Yufeng Fan,Cong-Yi Wang,Xiansheng Liu,Ruiping Zhang

iScience 26:107341 PubMed37539041

2023

GSDMA3 deficiency reprograms cellular metabolism and modulates BCR signaling in murine B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guan,Xi Luo,Ju Liu,Yanmei Huang,Qi Liu,Jiang Chang,Guofeng Fang,Danqing Kang,Heng Gu,Li Luo,Lu Yang,Zhaoyu Lin,Xiang Gao,Chaohong Liu,Jiahui Lei

The EMBO journal 39:e102783 PubMed31894880

2020

Cytoskeletal tension actively sustains the migratory T-cell synaptic contact.

Applications

Unspecified application

Species

Unspecified reactive species

Sudha Kumari,Michael Mak,Yeh-Chuin Poh,Mira Tohme,Nicki Watson,Mariane Melo,Erin Janssen,Michael Dustin,Raif Geha,Darrell J Irvine

Journal of leukocyte biology 103:867-883 PubMed29656494

2018

Oxidized LDL phagocytosis during foam cell formation in atherosclerotic plaques relies on a PLD2-CD36 functional interdependence.

Applications

Unspecified application

Species

Unspecified reactive species

Ramya Ganesan,Karen M Henkels,Lucile E Wrenshall,Yasunori Kanaho,Gilbert Di Paolo,Michael A Frohman,Julian Gomez-Cambronero

PLoS biology 11:e1001704 PubMed24223520

2013

N-wasp is essential for the negative regulation of B cell receptor signaling.

Applications

ICC/IF, ICC/IF, Flow Cyt, Flow Cyt

Species

Human, Mouse, Human, Mouse

Chaohong Liu,Xiaoming Bai,Junfeng Wu,Shruti Sharma,Arpita Upadhyaya,Carin I M Dahlberg,Lisa S Westerberg,Scott B Snapper,Xiaodong Zhao,Wenxia Song

Cancer research 73:3412-24 PubMed23644527

2013

CIP4 controls CCL19-driven cell steering and chemotaxis in chronic lymphocytic leukemia.

Applications

WB

Species

Human

Gema Malet-Engra,Julien Viaud,Loïc Ysebaert,Manon Farcé,Fanny Lafouresse,Guy Laurent,Frédérique Gaits-Iacovoni,Giorgio Scita,Loïc Dupré

Blood 120:3563-74 PubMed22932798

2012

Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules.

Applications

Unspecified application

Species

Unspecified reactive species

Sachin Kumar,Juying Xu,Charles Perkins,Fukun Guo,Scott Snapper,Fred D Finkelman,Yi Zheng,Marie-Dominique Filippi
View all publications
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