Rabbit Polyclonal WAVE 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Mouse Wasf1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Wasf1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8R5H6
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation (PubMed:29961568). The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). Also involved in the regulation of mitochondrial dynamics (PubMed:29961568).
Alternative names
KIAA0269, SCAR1, WAVE1, WASF1, Actin-binding protein WASF1, Protein WAVE-1, Verprolin homology domain-containing protein 1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1
Recommended products
Rabbit Polyclonal WAVE 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Mouse Wasf1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Wasf1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8R5H6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (ab272916)
4% PFA-fixed SK-N-SH (Human neuroblastoma cell line) cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).
Blue: Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (ab272916)
Paraffin-embedded mouse brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
Western blot - Anti-WAVE 1 antibody (ab272916)
All lanes: Western blot - Anti-WAVE 1 antibody (ab272916) at 1/1000 dilution
Lane 1: Mouse brain tissue extracts at 50 µg
Lane 2: Rat brain tissue extracts at 50 µg
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (ab272916)
4% PFA-fixed DIV9 rat E18 primary cortical neuron cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).
Red: Beta tubulin.
Blue: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (ab272916)
Paraffin-embedded rat brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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