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AB272916

Anti-WAVE 1 antibody

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(1 Publication)

Rabbit Polyclonal WAVE 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Wasf1.

View Alternative Names

KIAA0269, SCAR1, WAVE1, WASF1, Actin-binding protein WASF1, Protein WAVE-1, Verprolin homology domain-containing protein 1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (AB272916)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (AB272916)

4% PFA-fixed SK-N-SH (Human neuroblastoma cell line) cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).

Blue : Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (AB272916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (AB272916)

Paraffin-embedded mouse brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (AB272916)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (AB272916)

4% PFA-fixed DIV9 rat E18 primary cortical neuron cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).

Red : Beta tubulin.

Blue : DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (AB272916)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (AB272916)

Paraffin-embedded rat brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.

Western blot - Anti-WAVE 1 antibody (AB272916)
  • WB

Unknown

Western blot - Anti-WAVE 1 antibody (AB272916)

All lanes:

Western blot - Anti-WAVE 1 antibody (ab272916) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Wasf1. The exact immunogen used to generate this antibody is proprietary information.

Q8R5H6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation (PubMed : 29961568). The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). Also involved in the regulation of mitochondrial dynamics (PubMed : 29961568).
See full target information WASF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 30:9636897211048786 PubMed34606735

2021

Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Samaneh Khazaei,Masoud Soleimani,Seyed Hossein Ahmadi Tafti,Rouhollah Mehdinavaz Aghdam,Zohreh Hojati
View all publications
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Product promise

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