Rabbit Polyclonal WAVE 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Mouse Wasf1.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Mouse Wasf1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8R5H6
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation (PubMed:29961568). The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). Also involved in the regulation of mitochondrial dynamics (PubMed:29961568).
Alternative names
Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Protein WAVE-1, Verprolin homology domain-containing protein 1, WASF1, KIAA0269, SCAR1, WAVE1
Recommended products
Rabbit Polyclonal WAVE 1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Mouse Wasf1.
Alternative names
Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Protein WAVE-1, Verprolin homology domain-containing protein 1, WASF1, KIAA0269, SCAR1, WAVE1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Wasf1. The exact immunogen used to generate this antibody is proprietary information. Database link Q8R5H6
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (ab272916)
4% PFA-fixed SK-N-SH (Human neuroblastoma cell line) cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).
Blue: Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (ab272916)
Paraffin-embedded mouse brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
Western blot - Anti-WAVE 1 antibody (ab272916)
All lanes: Western blot - Anti-WAVE 1 antibody (ab272916) at 1/1000 dilution
Lane 1: Mouse brain tissue extracts at 50 µg
Lane 2: Rat brain tissue extracts at 50 µg
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WAVE 1 antibody (ab272916)
4% PFA-fixed DIV9 rat E18 primary cortical neuron cells stained for WAVE 1 using ab272916 at 1/500 dilution in ICC/IF (green).
Red: Beta tubulin.
Blue: DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WAVE 1 antibody (ab272916)
Paraffin-embedded rat brain tissue stained for WAVE1 using ab272916 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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