AB185546

Anti-WAVE 1 antibody [EPR15021] - N-terminal

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal WAVE 1 antibody. N-terminal. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0269, SCAR1, WAVE1, WASF1, Actin-binding protein WASF1, Protein WAVE-1, Verprolin homology domain-containing protein 1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1

2 Images

Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

Intracellular Flow Cytometry analysis of K562 cells labeling WAVE 1 using ab185546 at a 1/200 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Supplier Data

Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

All lanes:

Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (ab185546) at 1/10000 dilution

Lane 1:

U87-MG cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 61 kDa

Observed band size: 80 kDa

false

Carrier free

Anti-WAVE 1 antibody [EPR15021] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15021

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation (PubMed : 29961568). The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). Also involved in the regulation of mitochondrial dynamics (PubMed : 29961568).

See full target information WASF1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed39724071

2024

Tagless LysoIP for immunoaffinity enrichment of native lysosomes from clinical samples.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Saarela,Pawel Lis,Sara Gomes,Raja S Nirujogi,Wentao Dong,Eshaan Rawat,Sophie Glendinning,Karolina Zeneviciute,Enrico Bagnoli,Rotimi Fasimoye,Cindy Lin,Kwamina Nyame,Fanni A Boros,Friederike Zunke,Frederic Lamoliatte,Sadik Elshani,Matthew Jaconelli,Judith Jm Jans,Margriet A Huisman,Christian Posern,Lena M Westermann,Angela Schulz,Peter M van Hasselt,Dario R Alessi,Monther Abu-Remaileh,Esther M Sammler

Cell proliferation 55:e13169 PubMed34957619

2021

Glycomics reveal that ST6GAL1-mediated sialylation regulates uterine lumen closure during implantation.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Han,Feiyu Wang,Yulu Yue,Xihong Tan,Miao Tian,Yiliang Miao,Shuhong Zhao,Weijie Dong,Mei Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WAVE 1 antibody [EPR15021] - N-terminal

Flow Cytometry (Intracellular) - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

Western blot - Anti-WAVE 1 antibody [EPR15021] - N-terminal (AB185546)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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Product promise