Rabbit Polyclonal WBSCR22 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BUD23 aa 1-250.
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Publications
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- Microbiology and immunology 65:492-5042021BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBea Clarise B Garcia,Masayuki Horie,Shohei Kojima,Akiko Makino,Keizo TomonagaPubMed 34324219
- Molecular biology of the cell 26:2080-952015The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.Applications:WBReactive species:Unspecified reactive speciesChristiane Zorbas et. al.PubMed 25851604
- PloS one 8:e756862013The human WBSCR22 protein is involved in the biogenesis of the 40S ribosomal subunits in mammalian cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BUD23 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43709
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes The staining pattern is dependent on the antibody titer and nature of the biology. Proliferating tumor cells might be positive for nuclear staining while the differentiated cells show no nuclear staining. Antibody titer and antigen retrieval time should be adjusted. |
Target data
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA (PubMed:25851604). Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity (PubMed:25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed:25851604). Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity (PubMed:24086612). Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors (PubMed:24488492). Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements (PubMed:24488492). Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro (PubMed:24488492).
Alternative names
MERM1, WBSCR22, HUSSY-03, PP3381, BUD23, 18S rRNA (guanine-N(7))-methyltransferase, Bud site selection protein 23 homolog, Metastasis-related methyltransferase 1, Williams-Beuren syndrome chromosomal region 22 protein, rRNA methyltransferase and ribosome maturation factor
Recommended products
Rabbit Polyclonal WBSCR22 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BUD23 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BUD23 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link O43709
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-WBSCR22 antibody (ab97911)
12% SDS-PAGE.
All lanes: Western blot - Anti-WBSCR22 antibody (ab97911) at 1/500 dilution
Lane 1: Non-transfected lysate
Lane 2: 293T cell lysate transfected with WBSCR22
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBSCR22 antibody (ab97911)
ab97911 at 1/100 dilution staining WBSCR22 in CA922 xenograft by Immunohistochemistry, Paraffin-embedded tissue.
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