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AB97911

Anti-WBSCR22 antibody

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(3 Publications)

Rabbit Polyclonal WBSCR22 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BUD23 aa 1-250.

View Alternative Names

MERM1, WBSCR22, HUSSY-03, PP3381, BUD23, 18S rRNA (guanine-N(7))-methyltransferase, Bud site selection protein 23 homolog, Metastasis-related methyltransferase 1, Williams-Beuren syndrome chromosomal region 22 protein, rRNA methyltransferase and ribosome maturation factor

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBSCR22 antibody (AB97911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBSCR22 antibody (AB97911)

ab97911 at 1/100 dilution staining WBSCR22 in CA922 xenograft by Immunohistochemistry, Paraffin-embedded tissue.

Immunocytochemistry/ Immunofluorescence - Anti-WBSCR22 antibody (AB97911)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WBSCR22 antibody (AB97911)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling WBSCR22 protein at cytoplasm and nucleus using ab97911 at a 1/200 dilution (Green).
FIS1, a mitochondria marker, stained by FIS1 antibody at a 1/20000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBSCR22 antibody (AB97911)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBSCR22 antibody (AB97911)

Immunohistochemical analysis of mouse kidney tissue stained for WBSCR22 protein at cytoplasm with ab97911 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

Western blot - Anti-WBSCR22 antibody (AB97911)
  • WB

Supplier Data

Western blot - Anti-WBSCR22 antibody (AB97911)

Samples were separated by 12% SDS PAGE.

All lanes:

Western blot - Anti-WBSCR22 antibody (ab97911) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 30 µg

Lane 2:

HCT116 cell lysates at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BUD23 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

O43709

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of a guanine in 18S rRNA (PubMed : 25851604). Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity (PubMed : 25851604). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production independently of its RNA-modifying catalytic activity (PubMed : 25851604). Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity (PubMed : 24086612). Locus-specific steroid receptor coactivator. Potentiates transactivation by glucocorticoid (NR3C1), mineralocorticoid (NR3C2), androgen (AR) and progesterone (PGR) receptors (PubMed : 24488492). Required for the maintenance of open chromatin at the TSC22D3/GILZ locus to facilitate NR3C1 loading on the response elements (PubMed : 24488492). Required for maintenance of dimethylation on histone H3 'Lys-79' (H3K79me2), although direct histone methyltransferase activity is not observed in vitro (PubMed : 24488492).
See full target information BUD23

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Microbiology and immunology 65:492-504 PubMed34324219

2021

BUD23-TRMT112 interacts with the L protein of Borna disease virus and mediates the chromosomal tethering of viral ribonucleoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

Bea Clarise B Garcia,Masayuki Horie,Shohei Kojima,Akiko Makino,Keizo Tomonaga

Molecular biology of the cell 26:2080-95 PubMed25851604

2015

The human 18S rRNA base methyltransferases DIMT1L and WBSCR22-TRMT112 but not rRNA modification are required for ribosome biogenesis.

Applications

WB

Species

Unspecified reactive species

Christiane Zorbas,Emilien Nicolas,Ludivine Wacheul,Emmeline Huvelle,Valérie Heurgué-Hamard,Denis L J Lafontaine

PloS one 8:e75686 PubMed24086612

2013

The human WBSCR22 protein is involved in the biogenesis of the 40S ribosomal subunits in mammalian cells.

Applications

ICC/IF

Species

Human

Kadri Õunap,Ly Käsper,Ants Kurg,Reet Kurg
View all publications

Product promise

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