AB224221

Anti-WDHD1 antibody

Name: Anti-WDHD1 antibody (ab224221) | Abcam
Brand: Abcam Limited
SKU: AB224221
Price: 545 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal WDHD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDHD1 aa 200-350.

View Alternative Names

AND1, WDHD1, WD repeat and HMG-box DNA-binding protein 1, Acidic nucleoplasmic DNA-binding protein 1, And-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for WDHD1 (green) using ab224221 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

Paraffin-embedded human tonsil tissue stained for WDHD1 using ab224221 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-WDHD1 antibody (AB224221)

All lanes:

Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 126 kDa

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Supplier Data

Western blot - Anti-WDHD1 antibody (AB224221)

All lanes:

Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 126 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human WDHD1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

O75717

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core replisome component that acts as a replication initiation factor. Binds directly to the CMG complex and functions as a hub to recruit additional proteins to the replication fork.

See full target information WDHD1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of enzyme inhibition and medicinal chemistry 39:2383886 PubMed39072709

2024

Design, synthesis, and evaluation of novel stilbene derivatives that degrade acidic nucleoplasmic DNA-binding protein 1 (And1) and synergize with PARP1 inhibitor in NSCLC cells.

Applications

Unspecified application

Species

Unspecified reactive species

Leyuan Chen,Zhonghao Ren,Yunze Zhang,Wenbin Hou,Yiliang Li

FEBS open bio 13:102-117 PubMed36345604

2022

WDHD1 is over-expressed in nasopharyngeal carcinoma and may control the expression of ITGAV.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Yun Wu,Yi-Tong Niu,Su-Ning Huang,Yu-Min Tan,Zhen-Dong Yang,Ye-Ying Fang,Li Jiang,Ting-Ting Zhang,Xiao-Fen Zeng,Yun-Xi Peng,Miao Mo,Cai-Xing Lin,Zhu-Xin Wei

Nature communications 13:6090 PubMed36241664

2022

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri Lukas

Science (New York, N.Y.) 369: PubMed32973005

2020

The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Sue Im Sim,Alban Ordureau,Lianjie Wei,J Wade Harper,Sichen Shao,Eunyong Park

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDHD1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (AB224221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (AB224221)

Western blot - Anti-WDHD1 antibody (AB224221)

Western blot - Anti-WDHD1 antibody (AB224221)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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