Rabbit Polyclonal WDHD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDHD1 aa 200-350.
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Publications
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- FEBS open bio 13:102-1172022WDHD1 is over-expressed in nasopharyngeal carcinoma and may control the expression of ITGAV.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Yun Wu,Yi-Tong Niu,Su-Ning Huang,Yu-Min Tan,Zhen-Dong Yang,Ye-Ying Fang,Li Jiang,Ting-Ting Zhang,Xiao-Fen Zeng,Yun-Xi Peng,Miao Mo,Cai-Xing Lin,Zhu-Xin WeiPubMed 36345604
- Nature communications 13:60902022Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri LukasPubMed 36241664
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WDHD1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75717
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Core replisome component that acts as a replication initiation factor. Binds directly to the CMG complex and functions as a hub to recruit additional proteins to the replication fork.
Alternative names
AND1, WDHD1, WD repeat and HMG-box DNA-binding protein 1, Acidic nucleoplasmic DNA-binding protein 1, And-1
Recommended products
Rabbit Polyclonal WDHD1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDHD1 aa 200-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WDHD1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information. Database link O75717
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunocytochemistry/ Immunofluorescence - Anti-WDHD1 antibody (ab224221)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for WDHD1 (green) using ab224221 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDHD1 antibody (ab224221)
Paraffin-embedded human tonsil tissue stained for WDHD1 using ab224221 at 1/50 dilution in immunohistochemical analysis.
Western blot - Anti-WDHD1 antibody (ab224221)
All lanes: Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG sp (human brain glioma cell line) cell lysate
Lane 3: A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 126 kDa
Western blot - Anti-WDHD1 antibody (ab224221)
All lanes: Western blot - Anti-WDHD1 antibody (ab224221) at 1/250 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2: NBT-II cell lysate
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 126 kDa
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