Anti-WDR1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-WDR1 antibody (AB241366)

WDR1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241366 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241366 at 0.4 μg/ml.

Lane 1 : ab241366 IP in Jurkat whole cell lysate.

Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysate prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-WDR1 antibody (ab241366)

Predicted band size: 66 kDa

false

• WB

Supplier Data

Western blot - Anti-WDR1 antibody (AB241366)

All lanes:

Western blot - Anti-WDR1 antibody (ab241366) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 66 kDa

true

Exposure time: 30s