Rabbit Recombinant Monoclonal WDR1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.
Images
![Western blot - Anti-WDR1 antibody [EPR8793] (AB173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--western-blot-img28061.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR1 antibody [EPR8793] (AB173574)")
![Western blot - Anti-WDR1 antibody [EPR8793] (AB173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--western-blot-img30377.png/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR1 antibody [EPR8793] (AB173574)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (AB173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36195.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (AB173574)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (AB173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126640.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (AB173574)")
![Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (AB173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--immunocytochemistry-immunofluorescence-img62626.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (AB173574)")
Publications
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- Cancer science 112:893-9052020TNK2-AS1 upregulated by YY1 boosts the course of osteosarcoma through targeting miR-4319/WDR1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeitao Yao,Qiang Yan,Xinhui Du,Jingyu HouPubMed 33164271
- Scientific reports 8:130492018A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlfonso López-Coral et. al.PubMed 30158666
- Journal of neuroscience research 96:1798-18152018Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaved Iqbal et. al.PubMed 30054946
- PloS one 12:e01692602017Mapping the Interactome of a Major Mammalian Endoplasmic Reticulum Heat Shock Protein 90.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Hong et. al.PubMed 28056051
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted | Predicted |
| Chicken | Predicted | Not recommended | Not recommended | Predicted | Predicted |
| Cow | Predicted | Not recommended | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PubMed:15629458, PubMed:27557945, PubMed:29751004). Enhances cofilin-mediated actin severing (By similarity). Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions (PubMed:18494608). Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance (By similarity). Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension (By similarity). Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (PubMed:25792565).
Alternative names
WD repeat-containing protein 1, Actin-interacting protein 1, NORI-1, AIP1, WDR1
Recommended products
Rabbit Recombinant Monoclonal WDR1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR8793
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-WDR1 antibody [EPR8793] (ab173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--western-blot-img28061.png "Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)")
Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
Buffer concentration: 5% NFDM /TBST.
For human samples, please use lower antibody dilution or upload more of the protein.
All lanes: Western blot - Anti-WDR1 antibody [EPR8793] (ab173574) at 1/200 dilution
All lanes: Human heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 46 kDa
Exposure time: 180s
![Western blot - Anti-WDR1 antibody [EPR8793] (ab173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--western-blot-img30377.png "Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)")
Western blot - Anti-WDR1 antibody [EPR8793] (ab173574)
Buffer concentration: 5% NFDM /TBST
We are unsure as to the identity of the extra band below 75kDa.
All lanes: Western blot - Anti-WDR1 antibody [EPR8793] (ab173574) at 1/2000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 10 µg
Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg
Lane 3: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 10 µg
Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 75 kDa
Exposure time: 60s
Buffer concentration: 5% NFDM /TBST.
We are unsure as to the identity of the extra band below 75kDa.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36195.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126640.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] (ab173574)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574), expandable thumbnail](https://content.abcam.com/products/images/wdr1-antibody-epr8793-ab173574--immunocytochemistry-immunofluorescence-img62626.jpg "Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)")
Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] (ab173574)
Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).
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