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Rabbit Recombinant Monoclonal WDR1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

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Images

Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772), expandable thumbnail
  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772), expandable thumbnail
  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Predicted
Predicted
Not recommended
Not recommended
Predicted
Rat
Predicted
Predicted
Not recommended
Not recommended
Predicted
Chicken
Predicted
Predicted
Not recommended
Not recommended
Predicted
Cow
Predicted
Predicted
Not recommended
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow

Dilution info

-

Notes

-

Target data

Function

Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PubMed:15629458, PubMed:27557945, PubMed:29751004). Enhances cofilin-mediated actin severing (By similarity). Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions (PubMed:18494608). Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance (By similarity). Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension (By similarity). Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (PubMed:25792565).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal WDR1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR8793

Purification technique

Affinity purification

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab249772 is the carrier-free version of Anti-WDR1 antibody [EPR8793] ab173574.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772), expandable thumbnail

    Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772) at 1/1000 dilution

    Lane 1: Raji cell lysate at 10 µg

    Lane 2: U87-MG cell lysate at 10 µg

    Predicted band size: 66 kDa

  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772), expandable thumbnail

    Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    Buffer concentration: 5% NFDM /TBST

    For human samples, please use lower antibody dilution or upload more of the protein.

    All lanes: Western blot - Anti-WDR1 antibody [EPR8793] (Anti-WDR1 antibody [EPR8793] ab173574) at 1/200 dilution

    All lanes: Human heart tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 66 kDa

    Observed band size: 46 kDa

    Exposure time: 180s

  • Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772), expandable thumbnail

    Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    Buffer concentration: 5% NFDM /TBST.

    We are unsure as to the identity of the extra band below 75kDa.

    All lanes: Western blot - Anti-WDR1 antibody [EPR8793] (Anti-WDR1 antibody [EPR8793] ab173574) at 1/2000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

    Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

    Lane 3: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 10 µg

    Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 66 kDa

    Observed band size: 75 kDa

    Exposure time: 60s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with Anti-WDR1 antibody [EPR8793] ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with Anti-WDR1 antibody [EPR8793] ab173574 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772)

    This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Raji cells labeling WDR1 with Anti-WDR1 antibody [EPR8793] ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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