Rabbit Recombinant Monoclonal WDR1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | ICC/IF | IP | Flow Cyt | WB | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended | Tested |
Mouse | Predicted | Predicted | Not recommended | Not recommended | Predicted |
Rat | Predicted | Predicted | Not recommended | Not recommended | Predicted |
Chicken | Predicted | Predicted | Not recommended | Not recommended | Predicted |
Cow | Predicted | Predicted | Not recommended | Not recommended | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow | Dilution info - | Notes - |
Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (PubMed:15629458, PubMed:27557945, PubMed:29751004). Enhances cofilin-mediated actin severing (By similarity). Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions (PubMed:18494608). Involved in myocardium sarcomere organization. Required for cardiomyocyte growth and maintenance (By similarity). Involved in megakaryocyte maturation and platelet shedding. Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension (By similarity). Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (PubMed:25792565).
WD repeat-containing protein 1, Actin-interacting protein 1, NORI-1, AIP1, WDR1
Rabbit Recombinant Monoclonal WDR1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR8793
Affinity purification
Blue Ice
+4°C
+4°C
Do Not Freeze
ab249772 is the carrier-free version of Anti-WDR1 antibody [EPR8793] ab173574.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772) at 1/1000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: U87-MG cell lysate at 10 µg
Predicted band size: 66 kDa
Buffer concentration: 5% NFDM /TBST
For human samples, please use lower antibody dilution or upload more of the protein.
All lanes: Western blot - Anti-WDR1 antibody [EPR8793] (Anti-WDR1 antibody [EPR8793] ab173574) at 1/200 dilution
All lanes: Human heart tissue lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 46 kDa
Exposure time: 180s
Buffer concentration: 5% NFDM /TBST.
We are unsure as to the identity of the extra band below 75kDa.
All lanes: Western blot - Anti-WDR1 antibody [EPR8793] (Anti-WDR1 antibody [EPR8793] ab173574) at 1/2000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 10 µg
Lane 2: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg
Lane 3: HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 10 µg
Lane 4: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 75 kDa
Exposure time: 60s
This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with Anti-WDR1 antibody [EPR8793] ab173574 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with Anti-WDR1 antibody [EPR8793] ab173574 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using Anti-WDR1 antibody [EPR8793] ab173574, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of Raji cells labeling WDR1 with Anti-WDR1 antibody [EPR8793] ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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