Anti-WDR1 antibody [EPR8793] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772)

This data was developed using ab173574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772)

This data was developed using ab173574, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772)

This data was developed using ab173574, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772)

This data was developed using ab173574, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (ab249772) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Predicted band size: 66 kDa

false

• WB

Supplier Data

Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772)

Buffer concentration : 5% NFDM /TBST.

We are unsure as to the identity of the extra band below 75kDa.

All lanes:

Western blot - Anti-WDR1 antibody [EPR8793] (<a href='/en-us/products/primary-antibodies/wdr1-antibody-epr8793-ab173574'>ab173574</a>) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

Lane 3:

HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate at 10 µg

Lane 4:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 66 kDa

Observed band size: 75 kDa

false

Exposure time: 60s

• WB

Unknown

Western blot - Anti-WDR1 antibody [EPR8793] - BSA and Azide free (AB249772)

Buffer concentration : 5% NFDM /TBST

For human samples, please use lower antibody dilution or upload more of the protein.

All lanes:

Western blot - Anti-WDR1 antibody [EPR8793] (<a href='/en-us/products/primary-antibodies/wdr1-antibody-epr8793-ab173574'>ab173574</a>) at 1/200 dilution

All lanes:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 66 kDa

Observed band size: 46 kDa

false

Exposure time: 180s