AB252878

Anti-WDR12 antibody [1B8]

Recombinant
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(3 Publications)

Rat Recombinant Monoclonal WDR12 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Ribosome biogenesis protein WDR12, WD repeat-containing protein 12, WDR12

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling WDR12 with ab252878 at 12.35 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HeLa cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293 cells labelling WDR12 with ab252878 at 12.35 μg/mL, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing nucleolar staining in HEK-293 cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Lab

Western blot - Anti-WDR12 antibody [1B8] (AB252878)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : Lanes 1/3 : 3 seconds; Lane 2 : 15 seconds; Lane 4 : 1 second.

All lanes:

Western blot - Anti-WDR12 antibody [1B8] (ab252878) at 1.235 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 47 kDa

false

Carrier free

Anti-WDR12 antibody [1B8] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1B8

Isotype

IgG1

Light chain type

lambda

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1.235 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "12.35 µg/mL", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1.235 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1.235 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.

See full target information WDR12

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5294-5313 PubMed35672148

2022

Microglial mTOR Activation Upregulates Trem2 and Enhances β-Amyloid Plaque Clearance in the Alzheimer's Disease Model.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Shi,Cheng Chang,Afaf Saliba,Manzoor A Bhat

Frontiers in oncology 11:751792 PubMed34868955

2021

Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Lanjuan Mi,Qinghui Qi,Haowen Ran,Lishu Chen,Da Li,Dake Xiao,Jiaqi Wu,Yan Cai,Songyang Zhang,Yuanyuan Li,Bohan Li,Jiong Xie,Haohao Huang,Tao Li,Tao Zhou,Ailing Li,Ji Qi,Fangye Li,Jianghong Man

The Journal of cell biology 170:367-78 PubMed16043514

2005

Mammalian WDR12 is a novel member of the Pes1-Bop1 complex and is required for ribosome biogenesis and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hölzel,Michaela Rohrmoser,Martin Schlee,Thomas Grimm,Thomas Harasim,Anastassia Malamoussi,Anita Gruber-Eber,Elisabeth Kremmer,Wolfgang Hiddemann,Georg W Bornkamm,Dirk Eick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDR12 antibody [1B8]

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody [1B8] (AB252878)

Western blot - Anti-WDR12 antibody [1B8] (AB252878)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise