Rabbit Polyclonal WDR12 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR12 aa 1-300.
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Publications
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- Frontiers in oncology 11:7517922021Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanjuan Mi,Qinghui Qi,Haowen Ran,Lishu Chen,Da Li,Dake Xiao,Jiaqi Wu,Yan Cai,Songyang Zhang,Yuanyuan Li,Bohan Li,Jiong Xie,Haohao Huang,Tao Li,Tao Zhou,Ailing Li,Ji Qi,Fangye Li,Jianghong ManPubMed 34868955
- Nature communications 12:22592021SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowen Wang,Hong Zhang,Russell Sapio,Jun Yang,Justin Wong,Xin Zhang,Jessie Y Guo,Sharon Pine,Holly Van Remmen,Hong Li,Eileen White,Chen Liu,Megerditch Kiledjian,Dimitri G Pestov,X F Steven ZhengPubMed 33859191
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WDR12 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZL7
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
Alternative names
Ribosome biogenesis protein WDR12, WD repeat-containing protein 12, WDR12
Recommended products
Rabbit Polyclonal WDR12 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR12 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WDR12 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9GZL7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-WDR12 antibody (ab111955)
10% SDS PAGE
All lanes: Western blot - Anti-WDR12 antibody (ab111955) at 1/1000 dilution
All lanes: A431 whole cell lysate at 30 µg
Predicted band size: 17 kDa, 41 kDa, 42 kDa, 43 kDa, 46 kDa, 48 kDa, 49 kDa, 50 kDa, 53 kDa, 54 kDa
Western blot - Anti-WDR12 antibody (ab111955)
10% SDS PAGE
All lanes: Western blot - Anti-WDR12 antibody (ab111955) at 1/1000 dilution
All lanes: Mouse brain lysate at 30 µg
Predicted band size: 43 kDa, 48 kDa, 52 kDa, 67 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WDR12 antibody (ab111955)
ab111955 at 1/200 dilution staining WDR12 in paraformaldehyde-fixed A431 cells by Immunofluorescence. The lower image is costained with Hoechst 33342.
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