Rabbit Polyclonal WDR16 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CFAP52 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CFAP52 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N1V2
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme (PubMed:36191189). Important for proper ciliary and flagellar beating. May act in cooperation with CFAP45 and axonemal dynein subunit DNAH11 (PubMed:33139725). May play a role in cell growth and/or survival (PubMed:15967112).
Alternative names
WDR16, WDRPUH, CFAP52, Cilia- and flagella-associated protein 52, WD repeat-containing protein 16, WD40-repeat protein up-regulated in HCC
Recommended products
Rabbit Polyclonal WDR16 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CFAP52 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CFAP52 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N1V2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR16 also known as WD repeat domain 16 is a protein that is involved in several cellular processes through its structural role. It has a molecular mass of approximately 47 kDa and displays expression in various human tissues including the lungs brain and kidney. The protein belongs to the WD repeat protein family characterized by distinct WD repeat structures that facilitate protein-protein interactions.
- Biological function summary
WDR16 contributes to important cellular functions and it often participates as part of multiprotein complexes. The protein shows involvement in the regulation of ciliary functions aiding in the proper formation and maintenance of cilia. This role makes it significant in supporting cells' roles in movement fluid flow and sensory functions. Through this biological involvement WDR16 helps ensure proper communication and signaling within cells that contain cilia.
- Pathways
WDR16 plays a part in ciliary assembly and maintenance critical for the ciliogenesis pathway. This pathway involves several other proteins such as IFT20 which helps in the transport of ciliary components. Additionally WDR16 has interactions in the Wnt signaling pathway although its specific mechanisms are less thoroughly explored compared to its role in ciliary processes. These pathways emphasize WDR16's role in maintaining cellular structure and signaling required for normal cell function.
- Associated diseases and disorders
Mutations or dysfunction of WDR16 link to ciliopathies a group of disorders caused by defects in cilia structure and function. One such disease is nephronophthisis a kidney disorder characterized by inflammation and fibrosis. In this context WDR16 can connect with proteins like NEK8 which are implicated in similar pathways and disorders. Understanding these connections helps provide insight into the roles of WDR16 in disease pathology and potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-WDR16 antibody (ab127559)
7.5% SDS PAGE
All lanes: Western blot - Anti-WDR16 antibody (ab127559) at 1/2000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 68 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WDR16 antibody (ab127559)
ab127559, at a 1/500 dilution, staining WDR16 in methanol-fixed HeLa cells (green) by Confocal Immunofluorescence analysis (Olympus FV10i). Alpha tubulin filaments were labeled with an alpha tubulin antibody at a 1/2000 dilution (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR16 antibody (ab127559)
ab127559, at a 1/500 dilution, staining WDR16 in paraffin embedded CL15 xenograft by Immunohistochemistry.
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