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Rabbit Polyclonal WDR19 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WDR19 aa 1-250.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-WDR19 antibody (AB222908), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR19 antibody (AB222908), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR19 antibody (AB222908), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human WDR19 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NEZ3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/20 - 1/200
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/200
Notes

-

Associated Products

Select an associated product type

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Target data

Function

As component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in cilia function and/or assembly (PubMed:20889716). Essential for functional IFT-A assembly and ciliary entry of GPCRs (PubMed:20889716). Associates with the BBSome complex to mediate ciliary transport (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal WDR19 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WDR19 aa 1-250.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human WDR19 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NEZ3
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purity >95%

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

WDR19 also known as WD repeat-containing protein 19 or IFT144 plays an essential mechanical role in the intraflagellar transport system. This protein with a molecular mass of approximately 109 kDa facilitates movement of molecular cargo along axonemes within cilia. WDR19 expresses in ciliated cells across various tissues and contributes to ciliogenesis and maintenance of normal ciliary function. Its primary role involves interactions with other intraflagellar transport proteins aiding transportation processes within cilia.

Biological function summary

This protein contributes to the structure and function of motile and sensory cilian important components in cell signaling and motility. WDR19 operates as part of the IFT-A complex consisting of six proteins. These complexes help regulate the anterograde and retrograde transport essential for maintaining ciliary integrity. The precise interaction among IFT-A proteins allows efficient delivery and recycling of ciliary components thereby supporting cellular processes like developmental signaling and sensory perception.

Pathways

WDR19 participates extensively in the Hedgehog signaling pathway and the Wnt signaling pathway both important for development and differentiation. In Hedgehog signaling effective ciliary transport facilitated by WDR19 regulates the movement of morphogens necessary for patterning tissues during embryogenesis. Additionally its role in Wnt pathways helps modulate processes like cell fate determination. Within these pathways proteins such as IFT27 and IFT88 cooperate with WDR19 ensuring precise transport and signaling activities.

Associated diseases and disorders

Mutations in WDR19 can lead to ciliopathies including Sensenbrenner syndrome and nephronophthisis. Sensenbrenner syndrome presents with skeletal and craniofacial abnormalities due to disrupted ciliary function while nephronophthisis results in kidney disorders caused by dysfunctional cilia. WDR19 interacts with proteins like WDR35 and NPHP2 in these conditions which further establishes the disruptions in transport activities in ciliopathies. Understanding these interactions helps in identifying potential therapeutic targets to address these conditions.

Product promise

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3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR19 antibody (ab222908), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-WDR19 antibody (ab222908)

    A549 (human lung carcinoma cell line) cells stained for WDR19 (green) using ab222908 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR19 antibody (ab222908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR19 antibody (ab222908)

    Paraffin-embedded human liver cancer tissue stained for WDR19 using ab222908 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR19 antibody (ab222908), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR19 antibody (ab222908)

    Paraffin-embedded human placenta tissue stained for WDR19 using ab222908 at 1/100 dilution in immunohistochemical analysis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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