Anti-WDR19 antibody [EPR24915-11] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-WDR19 antibody [EPR24915-11] - BSA and Azide free (AB283709)

This data was developed using ab270970, the same antibody clone in a different buffer formulation.

WDR19 was immunoprecipitated from 0.35 mg mIMCD3 (mouse inner medullary collecting duct epithelial cell), whole cell lysate 10 ug with ab270970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270970 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : mIMCD3 (mouse inner medullary collecting duct epithelial cell), whole cell lysate 10 ug

Lane 2 : ab270970 IP in mIMCD3 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab270970 in mIMCD3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes

All lanes:

Immunoprecipitation - Anti-WDR19 antibody [EPR24915-11] (<a href='/en-us/products/primary-antibodies/wdr19-antibody-epr24915-11-ab270970'>ab270970</a>)

Predicted band size: 151 kDa

Observed band size: 150 kDa

false

• WB

Lab

Western blot - Anti-WDR19 antibody [EPR24915-11] - BSA and Azide free (AB283709)

This data was developed using ab270970, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-WDR19 antibody [EPR24915-11] (<a href='/en-us/products/primary-antibodies/wdr19-antibody-epr24915-11-ab270970'>ab270970</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

mIMCD3 (mouse inner medullary collecting duct epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 151 kDa

Observed band size: 150 kDa

false

• WB

Lab

Western blot - Anti-WDR19 antibody [EPR24915-11] - BSA and Azide free (AB283709)

This data was developed using ab270970, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Samples are non-boiled as boiling may cause protein aggregates.

Low expression : Spleen (PMID : 12906858)

Exposure time : Lane 1-2 : 26 seconds; Lane 3-6 : 103 seconds

All lanes:

Western blot - Anti-WDR19 antibody [EPR24915-11] (<a href='/en-us/products/primary-antibodies/wdr19-antibody-epr24915-11-ab270970'>ab270970</a>) at 1/1000 dilution

Lane 1:

Human epididymis tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat testis tissue lysate at 20 µg

Lane 6:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 151 kDa

Observed band size: 150 kDa

false