Rabbit Polyclonal WDR21A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCAF4 aa 50-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human DCAF4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WV16
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Alternative names
WDR21, WDR21A, DCAF4, DDB1- and CUL4-associated factor 4, WD repeat-containing protein 21A
Recommended products
Rabbit Polyclonal WDR21A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DCAF4 aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human DCAF4 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WV16
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR21A also known as STRAP or Mak10 acts as an important component of cellular processes such as gene expression and signal transduction. Mechanically the WDR21A protein features a series of WD40 repeats which facilitate protein-protein interactions. Its molecular weight stands around 39 kDa. Researchers identify an expression of WDR21A across various tissues with observable levels in the liver lung and kidney highlighting its essential presence in the human body.
- Biological function summary
Associated with cellular machinery WDR21A serves as a co-chaperone in complex regulatory mechanisms. It functions within the prefoldin complex contributing to the stabilization of nascent polypeptides and assisting with proper protein folding. This participation plays a direct role in maintaining cellular homeostasis and efficiency by preventing misfolded protein aggregation. Dysregulation within these processes can lead to cellular stress and malfunctioning pathways emphasizing the role of WDR21A in maintaining cellular order.
- Pathways
WDR21A plays a role in pathways like the TGF-beta signaling and mTOR pathways which are critical in regulating cell growth proliferation and differentiation. Within these pathways WDR21A interacts with proteins such as Smad4 and Raptor. These interactions showcase the integrated role of WDR21A in facilitating important cellular responses to external and internal cues thereby influencing various cellular outcomes and states.
- Associated diseases and disorders
Alterations in WDR21A expression have been linked to cancer particularly hepatocellular carcinoma and its involvement in neurodegenerative disorders such as Huntington's disease. The connection to cancer involves interactions with proteins like Smad4 impacting cell cycle regulation and apoptosis. In neurodegeneration WDR21A's relationship with Huntington protein highlights its impact on neuroprotective pathways emphasizing the importance of its regulation for preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-WDR21A antibody (ab130417)
Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling WDR21A with ab130417 shows positive staining in the nucleus but excluded from the nucleoli.
Western blot - Anti-WDR21A antibody (ab130417)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: RT-4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: TonsilAll lanes: Western blot - Anti-WDR21A antibody (ab130417)
Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR21A antibody (ab130417)
Immunohistochemical analysis of Formalin-fixed, paraffin embedded Human uterus, pre-menopause tissue labelled with WDR21A antibody at 1/200 dilution.
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