Rabbit Polyclonal WDR26 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human WDR26.
Images
Publications
Filter by
- Scientific reports 10:71382020Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShimaa H A Soliman et. al.PubMed 32346083
- Scientific reports 9:98642019The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew E R Maitland,Gabriel Onea,Christopher A Chiasson,Xu Wang,Jun Ma,Sarah E Moor,Kathryn R Barber,Gilles A Lajoie,Gary S Shaw,Caroline Schild-PoulterPubMed 31285494
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WDR26. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7D7
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Chicken | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Guinea pig | Predicted |
| Horse | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Rhesus monkey | Predicted |
| Xenopus tropicalis | Predicted |
| Zebrafish | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.4 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Rabbit | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
G-beta-like protein involved in cell signal transduction (PubMed:15378603, PubMed:19446606, PubMed:22065575, PubMed:23625927, PubMed:26895380, PubMed:27098453). Acts as a negative regulator in MAPK signaling pathway (PubMed:15378603). Functions as a scaffolding protein to promote G beta:gamma-mediated PLCB2 plasma membrane translocation and subsequent activation in leukocytes (PubMed:22065575, PubMed:23625927). Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a negative regulator of the canonical Wnt signaling pathway through preventing ubiquitination of beta-catenin CTNNB1 by the beta-catenin destruction complex, thus negatively regulating CTNNB1 degradation (PubMed:27098453). Serves as a scaffold to coordinate PI3K/AKT pathway-driven cell growth and migration (PubMed:26895380). Protects cells from oxidative stress-induced apoptosis via the down-regulation of AP-1 transcriptional activity as well as by inhibiting cytochrome c release from mitochondria (PubMed:19446606). Protects also cells by promoting hypoxia-mediated autophagy and mitophagy (By similarity).
Alternative names
CDW2, MIP2, PRO0852, WDR26, WD repeat-containing protein 26, CUL4- and DDB1-associated WDR protein 2, Myocardial ischemic preconditioning up-regulated protein 2
Recommended products
Rabbit Polyclonal WDR26 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human WDR26.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
- Synthetic Peptide within Human WDR26. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H7D7
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab85962 was affinity purified using an epitope specific to WDR26 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR26 also known as WD repeat domain 26 is part of the WD repeat protein family which is important in protein-protein interactions. It weighs roughly 109 kDa. WDR26 expresses in various tissues with significant levels observed in the brain and testis. The protein itself has a molecular architecture enriched with WD repeats which facilitates its involvement in cellular processes by mediating complex formation and signal transduction.
- Biological function summary
In the cellular environment WDR26 engages in regulating intracellular signaling pathways. It is a component of the STRIPAK complex which includes several proteins playing roles in synaptic and cellular signaling. The involvement of WDR26 in the STRIPAK complex as a scaffold aids in the correct assembly and localization of the complex impacting cellular processes such as apoptosis and proliferation.
- Pathways
WDR26 integrates into key signaling pathways like the MAPK signaling pathway. It interacts with other proteins such as STRN3 and MOB3 to modulate signals that affect gene expression growth and cell differentiation. The MAPK pathway in particular facilitates responses to growth factors stress signals and mitogens illustrating the protein's versatility in signal transduction.
- Associated diseases and disorders
Defects or dysregulation of WDR26 relate to intellectual disabilities and certain neurodevelopmental disorders. Research indicates that changes in WDR26 expression can disturb normal brain development and function often connecting with other proteins like MEK1 which also participates in neurodevelopment. These connections link WDR26 closely to neurological functions and highlight its potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-WDR26 antibody (ab85962)
All lanes: Western blot - Anti-WDR26 antibody (ab85962) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 220 kDa, 50 kDa, 80 kDa, 90 kDa
Exposure time: 3min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-WDR26 antibody (ab85962)
WDR26 western blot using anti-WDR26 antibody ab85962. Publication image and figure legend from Maitland, M. E. R., Onea, G., et al., 2019, Sci Rep, PubMed 31285494.
ab85962 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab85962 please see the product overview.
Characterization of the CTLH complex. (a) Schematic representation of the CTLH complex. The model is adapted from the yeast Gid complex26. Note that the position of muskelin in the complex has not been formally defined. (b) Subunits of the CTLH complex are present in RanBPM immunocomplexes. HEK293 whole cell extracts were incubated with a RanBPM antibody and immunoprecipitated. Immunoprecipitates were analyzed by Western blot with the indicated antibodies. IgG was used as a negative control. (c) WDR26 associates with the CTLH complex. Whole cell extracts were prepared from HeLa cells untransfected (−) or transfected with FLAG-tagged WDR26 (+). FLAG-WDR26 was immunoprecipitated with a FLAG antibody and immunoprecipitates were analyzed by Western blot with the indicated antibodies. (d) GID4 associates with CTLH complex. Whole cell extracts were prepared from HEK293 cells untransfected (−) or transfected with HA tagged GID4 (+). HA-GID4 was immunoprecipitated with an HA antibody and immunoprecipitates were analyzed by Western blot with the indicated antibodies.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com