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AB176817

Anti-WDR3 antibody

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(2 Publications)

Rabbit Polyclonal WDR3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human WDR3 aa 200-300.

View Alternative Names

WD repeat-containing protein 3, WDR3

2 Images
Immunoprecipitation - Anti-WDR3 antibody (AB176817)
  • IP

Supplier Data

Immunoprecipitation - Anti-WDR3 antibody (AB176817)

Detection of WDR3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176817 at 3 μg/mg lysate for IP (Lane 1). For WB detection ab176817 was used at 0.1 μg/ml (Lane 2) represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-WDR3 antibody (ab176817)

Predicted band size: 106 kDa

false

Western blot - Anti-WDR3 antibody (AB176817)
  • WB

Supplier Data

Western blot - Anti-WDR3 antibody (AB176817)

All lanes:

Western blot - Anti-WDR3 antibody (ab176817) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 106 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human WDR3 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UNX4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab176817 was affinity purified using an epitope specific to WDR3 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WD repeat domain 3 also known as WDR3 is a protein consisting of a series of WD repeat motifs. The protein weighs approximately 67 kDa and is mainly expressed in multiple tissues including the heart lung and liver. Mechanically WDR3 serves as a component of ribosomal biosynthesis. It interacts with other proteins to assist the assembly of ribosomal subunits participating in the nucleolar processing of pre-18S ribosomal RNA.
Biological function summary

WDR3 plays an essential role in the formation and maturity of ribosomes. It forms part of the ribonucleoprotein complex aiding in the production of ribosomal RNA which is critical for protein synthesis. Through its interaction with other proteins WDR3 facilitates the transport and processing of key nucleolar components maintaining cellular function. Its presence in the nucleolus suggests its involvement in cell growth and division linking it to fundamental cellular processes.

Pathways

WDR3 closely engages with the ribosome biogenesis pathway contributing significantly to the creation of functional ribosomes. It also associates with the p53 signaling pathway indicating its role in cell cycle regulation and apoptosis. Proteins such as NOP56 and UTP14A share connections with WDR3 within these pathways often co-regulating ribosomal gene transcription and processing activities.

WDR3 has potential implications in cancer due to its involvement in cell cycle and growth regulation. Altered expression of WDR3 can contribute to tumorigenesis by affecting ribosome production and cellular proliferation. Additionally defects in WDR3 function may relate to Diamond-Blackfan anemia a disorder characterized by failure in red blood cell production. Here its association with ribosomal protein genes like RPS19 points to its significance in hematopoietic tissue homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.
See full target information WDR3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 36:2302-2312 PubMed34459097

2021

Silencing of miR-497-5p inhibits cell apoptosis and promotes autophagy in Parkinson's disease by upregulation of FGF2.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Zhu,Hui Zhang,Jun Gao,Yun Xu

Journal of experimental & clinical cancer research : CR 40:88 PubMed33648545

2021

Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Su,Shikai Zhu,Kai Chen,Hongji Yang,Mingwu Tian,Qiang Fu,Ganggang Shi,Shijian Feng,Dianyun Ren,Xin Jin,Chong Yang
View all publications
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Product promise

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