AB72115

Anti-WDR33 antibody

Name: Anti-WDR33 antibody (ab72115) | Abcam
Brand: Abcam Limited
SKU: AB72115

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(2 Publications)

Rabbit Polyclonal WDR33 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human WDR33.

View Alternative Names

WDC146, WDR33, pre-mRNA 3' end processing protein WDR33, WD repeat-containing protein 33, WD repeat-containing protein of 146 kDa

2 Images

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Immunoprecipitation - Anti-WDR33 antibody (AB72115)

Detection of WDR33 by Western Blot of Immunprecipitate.
ab72115 at 1µg/ml staining WDR33 in HeLa whole cell lysate immunoprecipitated using ab72115 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-WDR33 antibody (ab72115)

Predicted band size: 146 kDa

false

Unknown

Western blot - Anti-WDR33 antibody (AB72115)

All lanes:

Western blot - Anti-WDR33 antibody (ab72115) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg/mL

Lane 2:

HeLa whole cell lysate at 15 µg/mL

Lane 3:

HeLa whole cell lysate at 5 µg/mL

Lane 4:

293T whole cell lysate at 50 µg/mL

Predicted band size: 146 kDa

Observed band size: 180 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human WDR33. The exact immunogen used to generate this antibody is proprietary information.

Q9C0J8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72115 was affinity purified using an epitope specific to WDR33 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR33 also known as WD repeat domain 33 is a protein that plays an important role in transcription and RNA processing. It weighs approximately 150 kDa. WDR33 is expressed in various tissues including the heart lung and neurons indicating its broad functional relevance across different biological systems. As part of the cellular machinery it functions in transcription regulation and RNA processing by interacting with other components of these systems.

Biological function summary

The function of WDR33 extends to involvement in the cleavage and polyadenylation specificity factor (CPSF) complex. This complex is responsible for the processing of pre-mRNA specifically in mediating 3' end formation through cleavage and polyadenylation. Such activity is important for gene expression regulation highlighting WDR33's significant role in cellular gene expression controls. The protein interactions within the CPSF complex enable precise regulation of mRNA influencing protein synthesis.

Pathways

WDR33 interacts within the polyadenylation pathway and contributes to RNA maturation and stability. It partners with other vital proteins like CPSF1 and CPSF3 playing a part in the polyadenylation signal recognition. This process is important for RNA stability and subsequent translation into functional proteins. Additionally WDR33 is involved in transcription termination linking it to foundational cellular processes necessary for proper genetic expression.

WDR33 has been linked to developmental and neurological conditions. Mutations affecting WDR33 or alterations in its associated pathways can result in genetic disorders impacting developmental processes. Furthermore its interaction with components like CPSF2 in pathways can influence neurological functions where dysregulation may contribute to neurodegenerative diseases. These associations underline WDR33 as a focal point in understanding certain pathologies and potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.

See full target information WDR33

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of the American Heart Association 13:e034257 PubMed39547961

2024

Fas Apoptosis Inhibitory Molecule 2 Inhibits Pathological Cardiac Hypertrophy by Regulating the MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Zhong,Minyu Wu,Heping Xie,Xu Chen,Jiayi Li,Zhisheng Duan,Hong Chen,Ziyou Liu,Wei Liao,Yijian Chen

Scientific reports 14:13989 PubMed38886371

2024

In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Jado,Michelle Dow,Krypton Carolino,Adam Klie,Gregory J Fonseca,Trey Ideker,Hannah Carter,Elizabeth A Winzeler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDR33 antibody

Immunoprecipitation - Anti-WDR33 antibody (AB72115)

Western blot - Anti-WDR33 antibody (AB72115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise