Anti-WDR41 antibody [EPR26180-218] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-WDR41 antibody [EPR26180-218] - BSA and Azide free (AB314861)

This data was developed using ab314860, the same antibody clone in a different buffer formulation.

WDR41 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab314860 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314860 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2 : ab314860 at 1/30 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 3 : Rabbit monoclonal lgG (ab172730) instead of ab314860 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-WDR41 antibody [EPR26180-218] (<a href='/en-us/products/primary-antibodies/wdr41-antibody-epr26180-218-ab314860'>ab314860</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/wdr41-antibody-epr26180-218-ab314860'>ab314860</a> at 1/30 IP in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

true

Exposure time: 119s

• WB

Supplier Data

Western blot - Anti-WDR41 antibody [EPR26180-218] - BSA and Azide free (AB314861)

This data was developed usingab314860, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : SK-BR-3 (PMID : 32394588). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-WDR41 antibody [EPR26180-218] (<a href='/en-us/products/primary-antibodies/wdr41-antibody-epr26180-218-ab314860'>ab314860</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 40 µg

Lane 2:

SK-BR-3 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,37 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-WDR41 antibody [EPR26180-218] - BSA and Azide free (AB314861)

This data was developed usingab314860, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The blots of lane 1-2 and lane 3 were incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) and Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG, respectively. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-WDR41 antibody [EPR26180-218] (<a href='/en-us/products/primary-antibodies/wdr41-antibody-epr26180-218-ab314860'>ab314860</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Hela transfected with siRNA specifically targeting WDR41 whole cell lysate at 20 µg

Lane 3:

Human testis tissue lysate at 40 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Lane 3:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 50 kDa,37 kDa

false

Exposure time: 180s