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AB168532

Anti-WDR45 antibody

5

(2 Reviews)

|

(2 Publications)

Mouse Polyclonal WDR45 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WDR45.

View Alternative Names

WDRX1, WDRXI4, WIPI4, JM5, WDR45, WD repeat domain phosphoinositide-interacting protein 4, WIPI-4, WD repeat-containing protein 45

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-WDR45 antibody (AB168532)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WDR45 antibody (AB168532)

Immunofluorescence analysis of HeLa cells, labeling WDR45 with ab168532 at 10μg/ml.

Western blot - Anti-WDR45 antibody (AB168532)
  • WB

Unknown

Western blot - Anti-WDR45 antibody (AB168532)

All lanes:

Western blot - Anti-WDR45 antibody (ab168532) at 1 µg/mL

Lane 1:

WDR45 transfected 293T lysate at 15 µL

Lane 2:

Non-transfected 293T lysate at 15 µL

Predicted band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human WDR45.

Q9Y484

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The WDR45 protein also known as WD repeat domain phosphoinositide-interacting protein 4 (WIPI4) plays a necessary role in the autophagic process. It is a protein of approximately 42 kDa and consists of seven WD40 repeats which facilitate protein-protein interactions. The protein is broadly expressed in various tissues particularly in the brain. These interactions are critical in autophagy a process for degrading and recycling cellular components.
Biological function summary

WDR45 contributes significantly to the regulation of autophagy. It acts as part of a multi-protein complex referred to as the autophagy-related 2 (ATG) complex. This complex is involved in autophagosome formation which is an important component in cellular degradation pathways. WDR45 binds phosphoinositides which are phospholipids integral to vesicle trafficking and membrane dynamics during autophagy.

Pathways

The influence of WDR45 is evident in the PI3K/Akt signaling and AMPK signaling pathways. These pathways regulate cellular energy homeostasis and the response to metabolic stress. PI3K/Akt and AMPK modulate the activity of the mTOR pathway which is known to suppress autophagy. WDR45 interacts with other autophagy-related proteins such as LC3 and ULK1 facilitating the initiation of the autophagic process.

The connection to WDR45 is pronounced in neurodegenerative diseases like beta-propeller protein-associated neurodegeneration (BPAN) and Parkinson's disease. Mutations in WDR45 lead to defective autophagy resulting in the accumulation of damaged organelles and proteins in neurons. It is linked to proteins involved in these disorders such as PINK1 and Parkin further highlighting its potential role in disease pathology through impaired mitochondrial quality control and neural damage.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation (PubMed : 23435086, PubMed : 28561066). Binds phosphatidylinositol 3-phosphate (PtdIns3P) (PubMed : 28561066). Activated by the STK11/AMPK signaling pathway upon starvation, WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PubMed : 28561066). Together with WIPI1, promotes ATG2 (ATG2A or ATG2B)-mediated lipid transfer by enhancing ATG2-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed : 31271352). Probably recruited to membranes through its PtdIns3P activity (PubMed : 28561066).
See full target information WDR45
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1272-1287 PubMed40715440

2025

Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Adriaenssens,Stefan Schaar,Annan S I Cook,Jan F M Stuke,Justyna Sawa-Makarska,Thanh Ngoc Nguyen,Xuefeng Ren,Martina Schuschnig,Julia Romanov,Grace Khuu,Louise Uoselis,Michael Lazarou,Gerhard Hummer,James H Hurley,Sascha Martens

eLife 11: PubMed35642785

2022

Differentiated glioma cell-derived fibromodulin activates integrin-dependent Notch signaling in endothelial cells to promote tumor angiogenesis and growth.

Applications

Unspecified application

Species

Unspecified reactive species

Shreoshi Sengupta,Mainak Mondal,Kaval Reddy Prasasvi,Arani Mukherjee,Prerna Magod,Serge Urbach,Dinorah Friedmann-Morvinski,Philippe Marin,Kumaravel Somasundaram
View all publications
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Product promise

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