Anti-WDR48/UAF1 antibody

Target data

Regulator of deubiquitinating complexes, which acts as a strong activator of USP1, USP12 and USP46 (PubMed : 18082604, PubMed : 19075014, PubMed : 26388029, PubMed : 31253762). Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself (PubMed : 18082604, PubMed : 31253762). Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate (PubMed : 19075014, PubMed : 27373336). Also activates deubiquitinating activity of complexes containing USP12 (PubMed : 19075014, PubMed : 27373336, PubMed : 27650958). In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability (PubMed : 24145035). Docks at the distal end of the USP12 fingers domain and induces a cascade of structural changes leading to the activation of the enzyme (PubMed : 27373336, PubMed : 27650958). Together with RAD51AP1, promotes DNA repair by stimulating RAD51-mediated homologous recombination (PubMed : 27239033, PubMed : 27463890, PubMed : 32350107). Binds single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) (PubMed : 27239033, PubMed : 31253762, PubMed : 32350107). DNA-binding is required both for USP1-mediated deubiquitination of FANCD2 and stimulation of RAD51-mediated homologous recombination : both WDR48/UAF1 and RAD51AP1 have coordinated role in DNA-binding during these processes (PubMed : 31253762, PubMed : 32350107). Together with ATAD5 and by regulating USP1 activity, has a role in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed : 20147293). Together with ATAD5, has a role in recruiting RAD51 to stalled forks during replication stress (PubMed : 31844045).. (Microbial infection) In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP (PubMed : 12196293). In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1 (PubMed : 18032488).