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Mouse Monoclonal WDR5 antibody. Suitable for ChIP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR5 aa 1-150.


Images

Flow Cytometry - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail
  • Western blot - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail
  • Western blot - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

pH: 7.4

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human WDR5 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P61964

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ChIPFlow CytWBICC/IF
Human
Expected
Tested
Tested
Tested

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Contributes to histone modification (PubMed:16600877, PubMed:16829960, PubMed:19103755, PubMed:19131338, PubMed:19556245, PubMed:20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed:16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:19103755, PubMed:20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).

Alternative names

Recommended products

Mouse Monoclonal WDR5 antibody. Suitable for ChIP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR5 aa 1-150.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human WDR5 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P61964
Clone number

2C2

Purity

Tissue culture supernatant

Light chain type

kappa

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.

Biological function summary

WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.

Pathways

WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.

Associated diseases and disorders

WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.

Product promise

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4 product images

  • Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail

    Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)

    Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail

    Western blot - Anti-WDR5 antibody [2C2] (ab56919)

    WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-WDR5 antibody [2C2] (ab56919)

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 μg/ml in ICC/IF.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail

    Western blot - Anti-WDR5 antibody [2C2] (ab56919)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/mL

    All lanes: Hs 181.Tes cell lysate

    Predicted band size: 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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