Anti-WDR5 antibody [2C2]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (AB56919)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 μg/ml in ICC/IF.

This image was generated using the ascites version of the product.

• Flow Cyt

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Flow Cytometry - Anti-WDR5 antibody [2C2] (AB56919)

Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

• WB

Supplier Data

Western blot - Anti-WDR5 antibody [2C2] (AB56919)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/mL

All lanes:

Hs 181.Tes cell lysate

Predicted band size: 36 kDa

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• WB

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Western blot - Anti-WDR5 antibody [2C2] (AB56919)

WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-WDR5 antibody [2C2] (ab56919)

Predicted band size: 36 kDa

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• WB

CiteAb

Western blot - Anti-WDR5 antibody [2C2] (AB56919)

WDR5 western blot using anti-WDR5 antibody [2C2] ab56919. Publication image and figure legend from Zhou, C., Zou, J., et al., 2016, Sci Rep, PubMed 27804957.

ab56919 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab56919 please see the product overview.

INO80 interacts with Wdr5 in MSC.(A) Wdr5 is decreased in INO80-silencing MSC compared with control MSC. (B) Interaction between INO80 and Wdr5. MSC lysates were sonicated and incubated with IgG or Ino80 or Wdr5 antibodies, and the presence of Ino80 or Wdr5 in the copurified proteins were detected with western blot.

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