Mouse Monoclonal WDR5 antibody. Suitable for ChIP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR5 aa 1-150.
Images
![Flow Cytometry - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--flow-cytometry-img122273.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-WDR5 antibody [2C2] (AB56919)")
![Western blot - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--western-blot-img153539.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR5 antibody [2C2] (AB56919)")
![Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--immunocytochemistry-immunofluorescence-img108420.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (AB56919)")
![Western blot - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--western-blot-img152617.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR5 antibody [2C2] (AB56919)")
![Western blot - Anti-WDR5 antibody [2C2] (AB56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--western-blot-img442148.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR5 antibody [2C2] (AB56919)")
Publications
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- Cell death & disease 13:7702022Histone methyltransferase MLL1 drives renal tubular cell apoptosis by p53-dependent repression of E-cadherin during cisplatin-induced acute kidney injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyun Zhang,Yingjie Guan,Jianan Zou,Xu Yang,Georgia Bayliss,Shougang ZhuangPubMed 36068197
- Molecular neurobiology 59:5104-51202022WDR5-HOTTIP Histone Modifying Complex Regulates Neural Migration and Dendrite Polarity of Pyramidal Neurons via Reelin Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinhan Ka,Hyung-Goo Kim,Woo-Yang KimPubMed 35672601
- Nature communications 13:18552022Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Zhou,Xinyi Zhu,Zhizhong Ye,Yong-Fei Wang,Chao Yao,Ning Xu,Mi Zhou,Jianyang Ma,Yuting Qin,Yiwei Shen,Yuanjia Tang,Zhihua Yin,Hong Xu,Yutong Zhang,Xiaoli Zang,Huihua Ding,Wanling Yang,Ya Guo,John B Harley,Bahram Namjou,Kenneth M Kaufman,Leah C Kottyan,Matthew T Weirauch,Guojun Hou,Nan ShenPubMed 35388006
- Cell reports 36:1095982021The related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through acetyltransferase-independent mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVeronique Fischer,Damien Plassard,Tao Ye,Bernardo Reina-San-Martin,Matthieu Stierle,Laszlo Tora,Didier DevysPubMed 34433046
- Journal of virology 95:2021Localization of the WD repeat-containing protein 5 to the Virion Assembly Compartment Facilitates Human Cytomegalovirus Assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Yang,YongXuan Yao,Hui Wu,Hong Yang,Xue-Hui Ma,Dong Li,Xian-Zhang Wang,Sheng-Nan Huang,Xuan Jiang,Shuang Cheng,Jin-Yan Sun,Zhen-Li Huang,CongJian Zhao,Michael A McVoy,Jin-Hyun Ahn,Wen-Bo Zeng,William J Britt,Sitang Gong,Min-Hua LuoPubMed 33504601
- Cancer letters 477:1-92020Ectopic HOTTIP expression induces noncanonical transactivation pathways to promote growth and invasiveness in pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChi Hin Wong,Chi Han Li,Qifang He,Stephen Lam Chan,Joanna Hung-Man Tong,Ka-Fai To,Li-Zhu Lin,Yangchao ChenPubMed 32120024
- Cancer cell 36:645-659.e82019HOTTIP lncRNA Promotes Hematopoietic Stem Cell Self-Renewal Leading to AML-like Disease in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuacheng Luo et. al.PubMed 31786140
- Oncology letters 17:103-1122019Downregulation of long noncoding RNA FENDRR predicts poor prognosis in renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang He,Guangzheng Zhong,Pei Wang,Chun Jiang,Ning Jiang,Jian HuangPubMed 30655744
- Frontiers in immunology 9:25122018Divergent lncRNA Regulates Transcription in T-Helper 2 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHunter R Gibbons et. al.PubMed 30420860
- Journal of cellular physiology 234:6244-62532018Mll-COMPASS complexes mediate H3K4me3 enrichment and transcription of the osteoblast master gene Runx2/p57 in osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdriana Rojas,Hugo Sepulveda,Berta Henriquez,Rodrigo Aguilar,Tatiana Opazo,Gino Nardocci,Fernando Bustos,Jane B Lian,Janet L Stein,Gary S Stein,Brigitte van Zundert,Andre J van Wijnen,Miguel L Allende,Martin MontecinoPubMed 30256410
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human WDR5 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P61964
Reactivity data
Select an application| ChIP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Contributes to histone modification (PubMed:16600877, PubMed:16829960, PubMed:19103755, PubMed:19131338, PubMed:19556245, PubMed:20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed:16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:19103755, PubMed:20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
Alternative names
BIG3, WDR5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein
Recommended products
Mouse Monoclonal WDR5 antibody. Suitable for ChIP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WDR5 aa 1-150.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human WDR5 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P61964
- Clone number
- 2C2
- Purity
- Tissue culture supernatant
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.
- Biological function summary
WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.
- Pathways
WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.
- Associated diseases and disorders
WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
![Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--flow-cytometry-img122273.jpg "Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)")
Flow Cytometry - Anti-WDR5 antibody [2C2] (ab56919)
Overlay histogram showing HeLA cells stained with ab56919 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56919, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (Mouse IgG2b [PLPV219] - Isotype Control ab91366, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
![Western blot - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--western-blot-img153539.jpg "Western blot - Anti-WDR5 antibody [2C2] (ab56919)")
Western blot - Anti-WDR5 antibody [2C2] (ab56919)
WDR5 antibody (ab56919) at 1μg/lane + Hs 181 S cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-WDR5 antibody [2C2] (ab56919)
Predicted band size: 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--immunocytochemistry-immunofluorescence-img108420.jpg "Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)")
Immunocytochemistry/ Immunofluorescence - Anti-WDR5 antibody [2C2] (ab56919)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for WDR5 (green) using ab56919 at 10 μg/ml in ICC/IF.
This image was generated using the ascites version of the product.
![Western blot - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--western-blot-img152617.jpg "Western blot - Anti-WDR5 antibody [2C2] (ab56919)")
Western blot - Anti-WDR5 antibody [2C2] (ab56919)
This image was generated using the ascites version of the product.All lanes: Western blot - Anti-WDR5 antibody [2C2] (ab56919) at 1 µg/mL
All lanes: Hs 181.Tes cell lysate
Predicted band size: 36 kDa
![Western blot - Anti-WDR5 antibody [2C2] (ab56919), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-2c2-ab56919--western-blot-img442148.jpg "Western blot - Anti-WDR5 antibody [2C2] (ab56919)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-WDR5 antibody [2C2] (ab56919)
WDR5 western blot using anti-WDR5 antibody [2C2] ab56919. Publication image and figure legend from Zhou, C., Zou, J., et al., 2016, Sci Rep, PubMed 27804957.
ab56919 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab56919 please see the product overview.
INO80 interacts with Wdr5 in MSC.(A) Wdr5 is decreased in INO80-silencing MSC compared with control MSC. (B) Interaction between INO80 and Wdr5. MSC lysates were sonicated and incubated with IgG or Ino80 or Wdr5 antibodies, and the presence of Ino80 or Wdr5 in the copurified proteins were detected with western blot.
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