Anti-WDR5 antibody [EPR11350] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

WDR5 was immunoprecipitated from 0.35 mg U937 whole cell lysate with ab307664 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307664 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : U937 (human histiocytic lymphoma monocyte) whole cell lysate Lane 2 : ab307664 IP in U937 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307664 in U937 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 24 seconds The IP experiment was performed by ab307664 using U937 cells. On the left the IP blot was probed with ab307664 and on the right the blot was probed by another anti-WDR5 antibody (ab178410)(1 : 1000 dilution).

Lane 2:

Immunoprecipitation - Anti-WDR5 antibody [EPR27033-6] (<a href='/en-us/products/primary-antibodies/wdr5-antibody-epr27033-6-ab307664'>ab307664</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

All lanes:

U937 (human histiocytic lymphoma monocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 24s

• WB

Supplier Data

Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

All lanes:

Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Saos-2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Unknown

Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)

All lanes:

Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 36 kDa

false