Rabbit Recombinant Monoclonal WDR5 antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.
Images
![Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--western-blot-img1058.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)")
![Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--western-blot-img65529.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)")
![Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--flow-cytometry-intracellular-img2416.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105200.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112443.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (AB178410)")
Publications
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- Acta cirurgica brasileira 38:e3862232023WD repeat domain 5 promotes the development of late-onset preeclampsia by activating nuclear factor kappa B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXudong Zhao,Fengyun Su,Fanhua Kong,Juan Su,Xiaojing Yang,Lei Li,Aihua Li,Qinwen LiPubMed 38055397
- International journal of molecular sciences 24:2023Wds-Mediated H3K4me3 Modification Regulates Lipid Synthesis and Transport in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesTujing Zhao,Min Wang,Zheng Li,Hao Li,Dongqin Yuan,Xing Zhang,Mengge Guo,Wenliang Qian,Daojun ChengPubMed 37047100
- The journal of obstetrics and gynaecology research 49:141-1532022miR-101-3p contributes to the progression of preeclampsia by suppressing WDR5-mediated proliferation and invasion of trophoblast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXudong Zhao,Fengyun Su,Fanhua Kong,Qing Guo,Xiuzhu Wang,Hong Cui,Qinwen Li,Wangmeng Zhang,Lei Li,Aihua LiPubMed 36324256
- Biomolecules 12:2022Inhibition of Wdr5 Attenuates Ang-II-Induced Fibroblast-to-Myofibroblast Transition in Cardiac Fibrosis by Regulating Mdm2/P53/P21 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiali Yuan,Hong Peng,Binfeng Mo,Chengye Yin,Guojian Fang,Yingze Li,Yuepeng Wang,Renhua Chen,Qunshan WangPubMed 36358925
- Cell death & disease 13:5322022Furin extracellularly cleaves secreted PTENα/β to generate C-terminal fragment with a tumor-suppressive role.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Zhang,Hong-Ming Ma,Shuang-Shu Dong,Na Zhang,Ping He,Meng-Kai Ge,Li Xia,Jian-Xiu Yu,Qiang Xia,Guo-Qiang Chen,Shao-Ming ShenPubMed 35668069
- Science advances 7:2021Structural insights into the recognition of histone H3Q5 serotonylation by WDR5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhao,Wanbiao Chen,Yi Pan,Yinfeng Zhang,Huiying Sun,Han Wang,Fan Yang,Yu Liu,Nan Shen,Xuan Zhang,Xi Mo,Jianye ZangPubMed 34144982
- Journal of hepatocellular carcinoma 8:333-3482021LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingying Zhang,Hongyu Zhang,Shuhuan WuPubMed 33977095
- Molecular therapy : the journal of the American Society of Gene Therapy 29:2134-21502021WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianli Chen,Kangshuai Li,Zengli Liu,Jialiang Liu,Yue Wang,Rongqi Sun,Zhipeng Li,Bo Qiu,Xiaoming Zhang,Guangli Ren,Yunfei Xu,Zongli ZhangPubMed 33601056
- Frontiers in neuroanatomy 14:362020Mixed Lineage Leukemia 1 Promoted Neuron Apoptosis in Ischemic Penumbra Regulating ASK-1/TNF-α Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhang Feng et. al.PubMed 32792914
- Oncology letters 15:7864-78702018Effect of high WDR5 expression on the hepatocellular carcinoma prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhoujun Cui et. al.PubMed 29731905
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested | Not recommended | Expected |
| Rat | Expected | Not recommended | Tested | Not recommended | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Contributes to histone modification (PubMed:19131338, PubMed:19556245, PubMed:19103755, PubMed:20018852, PubMed:16600877, PubMed:16829960). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed:16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:19103755, PubMed:20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
Alternative names
WD repeat-containing protein 5, BMP2-induced 3-kb gene protein, WDR5, BIG3
Recommended products
Rabbit Recombinant Monoclonal WDR5 antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 12 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR11350
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.
- Biological function summary
WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.
- Pathways
WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.
- Associated diseases and disorders
WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--western-blot-img1058.jpg "Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
All lanes: Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: Mouse spleen tissue lysate at 20 µg
Lane 3: Rat spleen tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
![Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--western-blot-img65529.jpg "Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
All lanes: Western blot - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Saos-2 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
![Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--flow-cytometry-intracellular-img2416.jpg "Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Flow Cytometry (Intracellular) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
Intracellular flow cytometrical analysis of permeabilized HeLa cells labeling WDR5 with ab178410 at 1/10 (red) compared to a nonspecific negative control antibody (green).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105200.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling WDR5 with ab178410 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112443.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
Immunohistochemical analysis of paraffin embedded Human breast tissue labeling WDR5 with ab178410 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--immunoprecipitation-img408594.jpg "Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)
WDR5 was immunoprecipitated from 0.35 mg U937 whole cell lysate with Anti-WDR5 antibody [EPR27033-6] ab307664 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-WDR5 antibody [EPR27033-6] ab307664 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: U937 (human histiocytic lymphoma monocyte) whole cell lysate
Lane 2: Anti-WDR5 antibody [EPR27033-6] ab307664 IP in U937 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-WDR5 antibody [EPR27033-6] ab307664 in U937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
The IP experiment was performed by Anti-WDR5 antibody [EPR27033-6] ab307664 using U937 cells. On the left the IP blot was probed with Anti-WDR5 antibody [EPR27033-6] ab307664 and on the right the blot was probed by another anti-WDR5 antibody (ab178410)(1:1000 dilution).Lane 2: Immunoprecipitation - Anti-WDR5 antibody [EPR27033-6] (Anti-WDR5 antibody [EPR27033-6] ab307664) at 1/30 dilution
Lane 2: Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410) at 1/1000 dilution
All lanes: U937 (human histiocytic lymphoma monocyte) whole cell lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 24s
![Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410), expandable thumbnail](https://content.abcam.com/products/images/wdr5-antibody-epr11350-c-terminal-ab178410--immunoprecipitation-img408594.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-WDR5 antibody [EPR11350] - C-terminal (ab178410)")
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