JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB154044

Anti-WDR62 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal WDR62 antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Wdr62 aa 750-800.

View Alternative Names

WD repeat-containing protein 62, Wdr62

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR62 antibody (AB154044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR62 antibody (AB154044)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for Wdr62 protein at nucleus with ab154044 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR62 antibody (AB154044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR62 antibody (AB154044)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for Wdr62 protein at nucleus with ab154044 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-WDR62 antibody (AB154044)
  • WB

Supplier Data

Western blot - Anti-WDR62 antibody (AB154044)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-WDR62 antibody (ab154044) at 1/1000 dilution

All lanes:

NIH-3T3 Whole cell extract at 30 µg

Predicted band size: 167 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Wdr62 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

Q3U3T8

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
NIH 3T3 whole cell lysate; Human Ca922 oral cancer xenograft tissue.
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR62 also known as WD repeat-containing protein 62 is a protein with a molecular mass of approximately 152 kDa. It localizes predominantly in the cytoplasm and the spindle poles of dividing cells. The protein contains several WD40 repeats which are motifs critical for protein-protein interactions. WDR62 is expressed in various tissues including the brain suggesting a significant function in neural development.
Biological function summary

WDR62 plays an essential role in neurogenesis by influencing cell proliferation and differentiation. It is a component of the spindle assembly complex ensuring proper chromosomal segregation during cell division. The protein is particularly active during mitosis where it participates in establishing spindle pole organization. Proper function of WDR62 is necessary for maintaining the integrity of the neuronal population in the developing brain.

Pathways

Spindle assembly and mitotic progression heavily involve WDR62. It interacts with Aurora A kinase an important player in the mitotic checkpoint pathway allowing accurate chromosome alignment and segregation. WDR62 also associates with c-Jun N-terminal kinase (JNK) signaling which impacts cell cycle regulation and apoptosis. These interactions suggest that WDR62 plays a role in ensuring normal cell division and viability.

Mutations in WDR62 often lead to microcephaly a condition characterized by reduced brain size and intellectual disability. This link highlights the protein's critical function in brain development. Additionally aberrations in WDR62 might contribute to epileptic syndromes reflecting its involvement in neuronal health. In these disorders it interacts with proteins such as ASPM and CEP152 which are also implicated in microcephaly and cortical malformations.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for cerebral cortical development. Plays a role in neuronal proliferation and migration (By similarity). Plays a role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and CEP63 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication (By similarity).
See full target information Wdr62
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 14:e1007463 PubMed30102701

2018

Wdr62 is involved in female meiotic initiation via activating JNK signaling and associated with POI in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhou,Yan Qin,Yingying Qin,Binyang Xu,Ting Guo,Hanni Ke,Min Chen,Lianjun Zhang,Feng Han,Yaqiong Li,Min Chen,Axel Behrens,Yaqing Wang,Zhiheng Xu,Zi-Jiang Chen,Fei Gao
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB245643

Anti-WDR62 antibody

Immunoprecipitation - Anti-WDR62 antibody (AB245643)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB255822

Anti-P-Selectin (CD62P) antibody [EPR22850-190]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P-Selectin (CD62P) antibody [EPR22850-190] (AB255822)

  • 0
  • 0
Proteins & Peptides

AB166176

Recombinant Human WDR62 protein

SDS-PAGE - Recombinant Human WDR62 protein (AB166176)

  • 0
  • 0
Primary Antibodies

AB247736

Anti-TRAF4 antibody [EPR1729] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-TRAF4 antibody [EPR1729] - BSA and Azide free (AB247736)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com