AB216126

Anti-WDR63 antibody

Name: Anti-WDR63 antibody (ab216126) | Abcam
Brand: Abcam Limited
SKU: AB216126

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(2 Publications )

Rabbit Polyclonal WDR63 antibody. Suitable for IHC-P, WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DNAI3 aa 400-550.

View Alternative Names

WDR63, DNAI3, Dynein axonemal intermediate chain 3, Testis development protein NYD-SP29, WD repeat-containing protein 63

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

Immunohistochemical analysis of paraffin-embedded Human fallopian tissue labeling WDR63 with ab216126 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling WDR63 with ab216126 at 1/200 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling WDR63 with ab216126 at 1/200 dilution.

Supplier Data

Western blot - Anti-WDR63 antibody (AB216126)

A tagged protein is being detected so the size will differ from that predicted.

All lanes:

Western blot - Anti-WDR63 antibody (ab216126) at 1/100 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

WDR63 over-expression Lysate (Co-expressed with a C-terminal myc-DDK).

Secondary

All lanes:

Swine anti-rabbit HRP

Predicted band size: 103 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DNAI3 aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

Q8IWG1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

The antibody solution should be gently mixed before use.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR63 also known as WD Repeat Domain 63 is a protein that plays a significant mechanical role in cellular processes. It is a protein which weighs approximately 97 kDa. This protein belongs to the WD repeat protein family characterized by repetitive sequences that aid in protein-protein interactions. WDR63 is expressed across various tissues with notable expression in the testis suggesting a role in reproductive biology.

Biological function summary

WDR63 participates in processes critical to cell structure and function. It associates with cellular structures that facilitate cell division. WDR63 is part of macromolecular complexes necessary for constructing cilium the antenna-like structures on the cell surface. Its involvement in such complexes aids in maintaining ciliary structure and function contributing to cellular signaling and movement.

Pathways

WDR63 has roles in pathways linked to ciliary function and cell cycle regulation. It is involved in the Hedgehog signaling pathway which is important for embryonic development and tissue homeostasis. This pathway interacts with proteins like SMO (Smoothened) and PTCH (Patched) regulating cellular responses to extracellular signals. Additionally WDR63 contributes to the centrosome cycle coordinating centrosome maturation and separation during cell division.

Research indicates links between WDR63 and specific ciliopathies. Mutations or dysregulation of WDR63 may contribute to conditions like Bardet-Biedl syndrome a disorder associated with impaired cilia function leading to issues in vision weight and renal function. WDR63 may interact with BBS1 and BBS10 proteins both relevant to the disorder. Understanding WDR63's role may offer insights into therapeutic strategies for ciliary-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Acts as a negative regulator of cell migration, invasion, and metastasis downstream of p53/TP53, through inhibition of Arp2/3 complex-mediated actin polymerization (PubMed : 32128961). Via its association with the multisubunit axonemal dynein complex, is potentially involved in the regulation of cilia function (By similarity). May play a role in osteogenesis of dental tissue-derived mesenchymal stem cells (By similarity).

See full target information DNAI3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 19:98 PubMed39696638

2024

Dementia with lewy bodies patients with high tau levels display unique proteome profiles.

Applications

Unspecified application

Species

Unspecified reactive species

Sinead Greally,Mukesh Kumar,Christoph Schlaffner,Hanne van der Heijden,Elisabeth S Lawton,Deeptarup Biswas,Sabina Berretta,Hanno Steen,Judith A Steen

EMBO reports 21:e49269 PubMed32128961

2020

WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kailiang Zhao,Decai Wang,Xiaolong Zhao,Chenfeng Wang,Yongxiang Gao,Kaiyue Liu,Fang Wang,Xianning Wu,Xuejuan Wang,Linfeng Sun,Jianye Zang,Yide Mei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDR63 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR63 antibody (AB216126)

Western blot - Anti-WDR63 antibody (AB216126)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise