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AB121771

Anti-WDR67 antibody

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(2 Publications)

Rabbit Polyclonal WDR67 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D31 aa 600-750.

View Alternative Names

WDR67, TBC1D31, TBC1 domain family member 31, WD repeat-containing protein 67

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-WDR67 antibody (AB121771)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WDR67 antibody (AB121771)

Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab121771 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR67 antibody (AB121771)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR67 antibody (AB121771)

ab121771 at 1/50 dilution staining WDR67 in paraffin embedded Human testis tissue by Immunohistochemistry.

Western blot - Anti-WDR67 antibody (AB121771)
  • WB

Supplier Data

Western blot - Anti-WDR67 antibody (AB121771)

All lanes:

Western blot - Anti-WDR67 antibody (ab121771)

Lane 1:

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10

Lane 2:

Negative control (vector only transfected HEK293T lysate)

Lane 3:

Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407919)

Predicted band size: 124 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TBC1D31 aa 600-750. The exact immunogen used to generate this antibody is proprietary information.

Q96DN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR67 also known as WD repeat-containing protein 67 is a protein involved in cellular mechanics with a mass of approximately 158 kDa. It localizes in the cytoplasm and nucleus indicating roles in different cellular processes. This protein belongs to the WD repeat protein family recognized for their role in forming protein-protein interactions. Evidence shows WDR67 is ubiquitously expressed in various tissues with higher levels in neuronal and muscular tissues suggesting functional importance in these areas.
Biological function summary

The function of WDR67 integrates into several cellular activities through its role as part of larger multiprotein complexes. These complexes participate in processes such as signal transduction and cell cycle progression. WDR67 interacts with other proteins to regulate protein assembly and cellular organization. This role lists it as an adapter or scaffold protein facilitating the correct localization and function of its binding partners within cells.

Pathways

WDR67 plays roles in the Wnt signaling and cell cycle control pathways. It acts by modulating these pathways affecting cellular growth and proliferation. It interacts with key signaling proteins such as beta-catenin within the Wnt pathway which is critical for regulating gene expression and maintaining cellular health. These interactions help modulate cellular responses to various developmental cues and stress conditions.

WDR67 is linked to Meckel-Gruber syndrome and Joubert syndrome suggesting a role in ciliopathy disorders. These disorders occur due to WDR67’s interactions with other ciliary proteins like MKS1. Mutations or disruptions in WDR67 can lead to improper functioning of cilia the cell's sensory and signaling structures contributing to the developmental abnormalities observed in these syndromes. Understanding its role can provide insights into the treatment or management of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Molecular adapter which is involved in cilium biogenesis. Part of a functional complex including OFD1 a centriolar protein involved in cilium assembly. Could regulate the cAMP-dependent phosphorylation of OFD1, and its subsequent ubiquitination by PJA2 which ultimately leads to its proteasomal degradation.
See full target information TBC1D31
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 40:e106503 PubMed33934390

2021

The TBC1D31/praja2 complex controls primary ciliogenesis through PKA-directed OFD1 ubiquitylation.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuela Senatore,Francesco Chiuso,Laura Rinaldi,Daniela Intartaglia,Rossella Delle Donne,Emilia Pedone,Bruno Catalanotti,Luciano Pirone,Bianca Fiorillo,Federica Moraca,Giuliana Giamundo,Giovanni Scala,Andrea Raffeiner,Omar Torres-Quesada,Eduard Stefan,Marcel Kwiatkowski,Alienke van Pijkeren,Manuela Morleo,Brunella Franco,Corrado Garbi,Ivan Conte,Antonio Feliciello

The Journal of allergy and clinical immunology 130:481-8.e2 PubMed22721650

2012

LPS-responsive beige-like anchor (LRBA) gene mutation in a family with inflammatory bowel disease and combined immunodeficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Abdullah Alangari,Abdulrahman Alsultan,Nouran Adly,Michel J Massaad,Iram Shakir Kiani,Abdulrahman Aljebreen,Emad Raddaoui,Abdul-Kareem Almomen,Saleh Al-Muhsen,Raif S Geha,Fowzan S Alkuraya
View all publications
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