Rabbit Polyclonal WDR67 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D31 aa 600-750.
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- The EMBO journal 40:e1065032021The TBC1D31/praja2 complex controls primary ciliogenesis through PKA-directed OFD1 ubiquitylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmanuela Senatore,Francesco Chiuso,Laura Rinaldi,Daniela Intartaglia,Rossella Delle Donne,Emilia Pedone,Bruno Catalanotti,Luciano Pirone,Bianca Fiorillo,Federica Moraca,Giuliana Giamundo,Giovanni Scala,Andrea Raffeiner,Omar Torres-Quesada,Eduard Stefan,Marcel Kwiatkowski,Alienke van Pijkeren,Manuela Morleo,Brunella Franco,Corrado Garbi,Ivan Conte,Antonio FelicielloPubMed 33934390
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TBC1D31 aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q96DN5
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Molecular adapter which is involved in cilium biogenesis. Part of a functional complex including OFD1 a centriolar protein involved in cilium assembly. Could regulate the cAMP-dependent phosphorylation of OFD1, and its subsequent ubiquitination by PJA2 which ultimately leads to its proteasomal degradation.
Alternative names
WDR67, TBC1D31, TBC1 domain family member 31, WD repeat-containing protein 67
Recommended products
Rabbit Polyclonal WDR67 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TBC1D31 aa 600-750.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TBC1D31 aa 600-750. The exact immunogen used to generate this antibody is proprietary information. Database link Q96DN5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR67 also known as WD repeat-containing protein 67 is a protein involved in cellular mechanics with a mass of approximately 158 kDa. It localizes in the cytoplasm and nucleus indicating roles in different cellular processes. This protein belongs to the WD repeat protein family recognized for their role in forming protein-protein interactions. Evidence shows WDR67 is ubiquitously expressed in various tissues with higher levels in neuronal and muscular tissues suggesting functional importance in these areas.
- Biological function summary
The function of WDR67 integrates into several cellular activities through its role as part of larger multiprotein complexes. These complexes participate in processes such as signal transduction and cell cycle progression. WDR67 interacts with other proteins to regulate protein assembly and cellular organization. This role lists it as an adapter or scaffold protein facilitating the correct localization and function of its binding partners within cells.
- Pathways
WDR67 plays roles in the Wnt signaling and cell cycle control pathways. It acts by modulating these pathways affecting cellular growth and proliferation. It interacts with key signaling proteins such as beta-catenin within the Wnt pathway which is critical for regulating gene expression and maintaining cellular health. These interactions help modulate cellular responses to various developmental cues and stress conditions.
- Associated diseases and disorders
WDR67 is linked to Meckel-Gruber syndrome and Joubert syndrome suggesting a role in ciliopathy disorders. These disorders occur due to WDR67’s interactions with other ciliary proteins like MKS1. Mutations or disruptions in WDR67 can lead to improper functioning of cilia the cell's sensory and signaling structures contributing to the developmental abnormalities observed in these syndromes. Understanding its role can provide insights into the treatment or management of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-WDR67 antibody (ab121771)
All lanes: Western blot - Anti-WDR67 antibody (ab121771)
Lane 1: Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407919)
Predicted band size: 124 kDa
Immunocytochemistry/ Immunofluorescence - Anti-WDR67 antibody (ab121771)
Immunofluorescent staining of Human cell line U-251MG shows positivity in centrosome. Recommended concentration of ab121771 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR67 antibody (ab121771)
ab121771 at 1/50 dilution staining WDR67 in paraffin embedded Human testis tissue by Immunohistochemistry.
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