AB190268

Anti-WDR72 antibody - C-terminal

Name: Anti-WDR72 antibody - C-terminal (ab190268) | Abcam
Brand: Abcam Limited
SKU: AB190268

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal WDR72 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human WD repeat-containing protein 72 aa 950-1100.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR72 antibody - C-terminal (AB190268)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR72 antibody - C-terminal (AB190268)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling WDR72 in the nucleus of cells in seminiferus ducts with ab190268 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human WD repeat-containing protein 72 aa 950-1100. The exact immunogen used to generate this antibody is proprietary information.

Q3MJ13

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR72 also known as WD repeat domain 72 is a protein that mechanically functions as a scaffolding unit. This protein enables the assembly of multi-protein complexes essential for maintaining cellular homeostasis. WDR72 has a molecular mass of approximately 110 kDa. It is expressed widely in tissues with significant levels found in the kidneys liver and the brain. This distribution suggests its importance in diverse bodily processes.

Biological function summary

Four roles that the WDR72 protein is involved in include processes related to enamel formation cellular signaling and vesicle transport. WDR72 contributes as a part of a protein complex that regulates ameloblast differentiation which directly impacts enamel development. By facilitating vesicle movement within cells it helps maintain proper cellular activity particularly in the matrix formation of dental tissues.

Pathways

Four key contributions in cellular signaling and enamel development rely on WDR72 functionality. It plays a role in the extracellular signal-regulated kinase (ERK) pathway important for transmitting signals from the cell membrane to the nucleus affecting cell division and survival. WDR72 is linked with proteins such as AMEL and ENAM which are significant within the pathway regulating tooth mineralization and calcification processes essential for strong enamel formation.

Four significant connections exist between WDR72 mutations and amelogenesis imperfecta a hereditary condition marked by defective enamel formation. This connection implicates WDR72 in dental health and integrity. Additionally malfunction or aberrations in WDR72 can relate to renal pathologies due to its expression in kidneys. Within the context of amelogenesis imperfecta WDR72 is connected with AMBN an ameloblast-associated protein highlighting their interaction during enamel formation and pathology when disrupted.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Plays a major role in formation of tooth enamel (PubMed : 19853237, PubMed : 25008349). Specifically required during the maturation phase of amelogenesis for normal formation of the enamel matrix and clearance of enamel proteins. May be involved in localization of the calcium transporter SLC24A4 to the ameloblast cell membrane.

See full target information WD repeat-containing protein 72

Alternative Names

WD repeat-containing protein 72, WDR72

style

left-column

Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Nature 633:895-904 PubMed39169180

2024

Short-term post-fast refeeding enhances intestinal stemness via polyamines.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Imada,Saleh Khawaled,Heaji Shin,Sven W Meckelmann,Charles A Whittaker,Renan Oliveira Corrêa,Chiara Alquati,Yixin Lu,Guodong Tie,Dikshant Pradhan,Gizem Calibasi-Kocal,Luiza Martins Nascentes Melo,Gabriele Allies,Jonas Rösler,Pia Wittenhofer,Jonathan Krystkiewicz,Oliver J Schmitz,Jatin Roper,Marco Aurelio Ramirez Vinolo,Luigi Ricciardiello,Evan C Lien,Matthew G Vander Heiden,Ramesh A Shivdasani,Chia-Wei Cheng,Alpaslan Tasdogan,Ömer H Yilmaz

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WDR72 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR72 antibody - C-terminal (AB190268)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (1)

Product promise