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AB154190

Anti-WDR77 antibody [EPR10708(B)]

5

(2 Reviews)

|

(8 Publications)

Rabbit Recombinant Monoclonal WDR77 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

MEP50, WD45, HKMT1069, Nbla10071, WDR77, Methylosome protein WDR77, Androgen receptor cofactor p44, Methylosome protein 50, WD repeat-containing protein 77, p44/Mep50, MEP-50

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (AB154190)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (AB154190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling WDR77 with purified ab154190 at 1/6000 dilution (0.05 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (AB154190)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10708(B)] (AB154190)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue sections labeling WDR77 with purified ab154190 at 1/6000 dilution (0.05 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (AB154190)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10708(B)] (AB154190)

Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling WDR77 with purified ab154190 at 1/50 dilution (5.58 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (AB154190)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10708(B)] (AB154190)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling WDR77 with purified ab154190 at 1/30 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Western blot - Anti-WDR77 antibody [EPR10708(B)] (AB154190)
  • WB

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Western blot - Anti-WDR77 antibody [EPR10708(B)] (AB154190)

All lanes:

Western blot - Anti-WDR77 antibody [EPR10708(B)] (ab154190) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Mouse spleen lysate

Lane 4:

Mouse kidney lysate

Lane 5:

Rat kidney lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

false

  • Carrier free

    Anti-WDR77 antibody [EPR10708(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10708(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/6000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/50 - 1/100.</strong></p><p>The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/30", "FlowCytIntra-species-notes": "<p>For unpurified use at 1/100 - 1/10000. <a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR77 also known as MEP50 is a protein with a mass of about 37 kDa. It functions as a cofactor involved in methylation processes often forming part of protein complexes with PRMT5. WDR77 locates within the cell nucleus and is widely expressed in human tissues with higher levels observed in reproductive organs like the testis and prostate. The protein interacts with other proteins to affect transcription and RNA processing.
Biological function summary

WDR77 interacts with the protein arginine methyltransferase 5 (PRMT5) and contributes to the assembly and stabilization of methyltransferase complexes. This role is essential for its participation in the methylation of arginine residues on histones and other proteins which regulates gene expression. WDR77 influences cell proliferation and differentiation by modulating these processes especially in germ cells and prostate epithelial cells.

Pathways

WDR77 is an important player in the transcriptional repression pathway affecting histone modification. It connects intimately with important pathways like the PI3K/AKT signaling and cell cycle regulation. Through its interaction with PRMT5 WDR77 takes part in regulatory networks impacting gene silencing chromatin remodeling and the overall gene expression mechanism. The involvement of WDR77 in these pathways emphasizes its relationship with PRMT5 and other methylation-associated proteins.

WDR77 has links to prostate cancer and lung cancer. These associations may result from its role in cell cycle control and proliferation which becomes dysregulated in oncogenesis. WDR77's interaction with PRMT5 is critical in such conditions as changes in their activity can alter normal cell growth patterns. Research indicates that abnormal expression or function of WDR77 can lead to increased tumorigenesis in specific tissues emphasizing the protein's potential as a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed : 11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed : 23071334).
See full target information WDR77
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 15:1529657 PubMed40510136

2025

METTL3-mediated SNHG1 mA modification promotes proliferation and migration through transcriptional regulation of WDR74 in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guanzhen Qiu,Yuxin Bao,Yuanzhuang Zhang,Yeqiu Xu,Tianhua Qiao,Chenghao Li,Hanjie Zhai,Zhenjun Chen,Fu Ren,Yong Wang

Translational oncology 49:102089 PubMed39182364

2024

WDR77 in Pan-Cancer: Revealing expression patterns, genetic insights, and functional roles across diverse tumor types, with a spotlight on colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Qihui Wu,Jiaxin Liu,Xuan Wang,Jialing Xie,Xiaodan Fu,Yimin Li

Communications biology 7:593 PubMed38760429

2024

PRMT5-mediated methylation of STAT3 is required for lung cancer stem cell maintenance and tumour growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Abe,Takumi Sano,Naoki Otsuka,Masashi Ogawa,Nobuyuki Tanaka

Nature communications 14:4824 PubMed37563140

2023

WDR77 inhibits prion-like aggregation of MAVS to limit antiviral innate immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxin Li,Rui Zhang,Changwan Wang,Junyan Zhu,Miao Ren,Yingbo Jiang,Xianteng Hou,Yangting Du,Qing Wu,Shishi Qi,Lin Li,She Chen,Hui Yang,Fajian Hou

eLife 10: PubMed34448450

2021

PRMT5 regulates ovarian follicle development by facilitating translation.

Applications

Unspecified application

Species

Unspecified reactive species

Min Chen,Fangfang Dong,Min Chen,Zhiming Shen,Haowei Wu,Changhuo Cen,Xiuhong Cui,Shilai Bao,Fei Gao

Biochemical and biophysical research communication 514:1185-1191 PubMed31103260

2019

The N-terminal sequence of murine PRMT5 variant 2 is required for Hsp70 interaction and CHIP ligase-mediated degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Weizhe Lu,Jun-Dal Kim,Saori Tabara,Chulwon Kwon,Hayase Mizukami,Keiji Kimura,Akiyoshi Fukamizu

The Journal of biological chemistry 293:17769-17779 PubMed30282801

2018

Sirtuin 7-mediated deacetylation of WD repeat domain 77 (WDR77) suppresses cancer cell growth by reducing WDR77/PRMT5 transmethylase complex activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Qi,Xiaoyan Shi,Miao Yu,Boya Liu,Minghui Liu,Shi Song,Shuaiyi Chen,Junhua Zou,Wei-Guo Zhu,Jianyuan Luo

PloS one 12:e0186982 PubMed29065155

2017

PRMT5: A novel regulator of Hepatitis B virus replication and an arginine methylase of HBV core.

Applications

WB

Species

Human

Barbora Lubyova,Jan Hodek,Ales Zabransky,Hana Prouzova,Martin Hubalek,Ivan Hirsch,Jan Weber
View all publications
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