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Rabbit Recombinant Monoclonal WDR77 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023), expandable thumbnail
  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023), expandable thumbnail
  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023), expandable thumbnail
  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones (PubMed:11756452). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:23071334).

Alternative names

MEP50, WD45, HKMT1069, Nbla10071, WDR77, Methylosome protein WDR77, Androgen receptor cofactor p44, Methylosome protein 50, WD repeat-containing protein 77, p44/Mep50, MEP-50

Recommended products

Rabbit Recombinant Monoclonal WDR77 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR10709(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251023 is the carrier-free version of Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

WDR77 also known as MEP50 is a protein with a mass of about 37 kDa. It functions as a cofactor involved in methylation processes often forming part of protein complexes with PRMT5. WDR77 locates within the cell nucleus and is widely expressed in human tissues with higher levels observed in reproductive organs like the testis and prostate. The protein interacts with other proteins to affect transcription and RNA processing.

Biological function summary

WDR77 interacts with the protein arginine methyltransferase 5 (PRMT5) and contributes to the assembly and stabilization of methyltransferase complexes. This role is essential for its participation in the methylation of arginine residues on histones and other proteins which regulates gene expression. WDR77 influences cell proliferation and differentiation by modulating these processes especially in germ cells and prostate epithelial cells.

Pathways

WDR77 is an important player in the transcriptional repression pathway affecting histone modification. It connects intimately with important pathways like the PI3K/AKT signaling and cell cycle regulation. Through its interaction with PRMT5 WDR77 takes part in regulatory networks impacting gene silencing chromatin remodeling and the overall gene expression mechanism. The involvement of WDR77 in these pathways emphasizes its relationship with PRMT5 and other methylation-associated proteins.

Associated diseases and disorders

WDR77 has links to prostate cancer and lung cancer. These associations may result from its role in cell cycle control and proliferation which becomes dysregulated in oncogenesis. WDR77's interaction with PRMT5 is critical in such conditions as changes in their activity can alter normal cell growth patterns. Research indicates that abnormal expression or function of WDR77 can lead to increased tumorigenesis in specific tissues emphasizing the protein's potential as a target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361) at 1/10000 dilution

    Lane 1: LnCaP cell lysate at 20 µg

    Lane 2: 293T cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa

    Observed band size: 42 kDa

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361) at 1/1000 dilution

    All lanes: Saos-2 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa

    Observed band size: 42 kDa

  • Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: RAW 264.7 cell lysate at 10 µg

    Lane 3: PC-12 cell lysate at 10 µg

    Lane 4: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa

    Observed band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.Immunoprecipitation of HeLa cells labeling WDR77 with Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 (+) or PBS (-)

    All lanes: Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    Predicted band size: 37 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.

  • Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

    This data was developed using Anti-WDR77 antibody [EPR10709(2)] - C-terminal ab190361, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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