Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling WDR77 with ab190361 at 1/50 and HRP polymer for Rabbit IgG. Counterstained with Hematoxylin. Negative control also shown.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of A673 cells fixed in 4% paraformaldehyde labeling WDR77 with ab190361 at 1/50 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) 1/200. Counterstained with DAPI. Negative controls also shown.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling WDR77 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150. Rabbit monoclonal IgG used as isotype control.

• IP

Unknown

Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

Immunoprecipitation of HeLa cells labeling WDR77 with ab190361 at 1/50 and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/5000 (+) or PBS (-)

All lanes:

Immunoprecipitation - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (ab251023)

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (<a href='/en-us/products/primary-antibodies/wdr77-antibody-epr107092-c-terminal-ab190361'>ab190361</a>) at 1/10000 dilution

Lane 1:

LnCaP cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 42 kDa

false

• WB

Supplier Data

Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (<a href='/en-us/products/primary-antibodies/wdr77-antibody-epr107092-c-terminal-ab190361'>ab190361</a>) at 1/1000 dilution

All lanes:

Saos-2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 42 kDa

false

• WB

Supplier Data

Western blot - Anti-WDR77 antibody [EPR10709(2)] - BSA and Azide free (AB251023)

This data was developed using ab190361, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WDR77 antibody [EPR10709(2)] - C-terminal (<a href='/en-us/products/primary-antibodies/wdr77-antibody-epr107092-c-terminal-ab190361'>ab190361</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 37 kDa

Observed band size: 42 kDa

false