Rabbit Polyclonal WDR79 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WRAP53 aa 100-250.
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- Histology and histopathology 39:877-8872023Selenomethionine suppresses head and neck squamous cell carcinoma progression through TopBP1/ATR and TCAB1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Zhang,Xiaodong Wei,Jiwu LiPubMed 37750664
- Open medicine (Warsaw, Poland) 18:202206242023WDR79 promotes aerobic glycolysis of pancreatic ductal adenocarcinoma (PDAC) by the suppression of SIRT4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenke Yin,Xiaoyan Song,Yue XiangPubMed 36712589
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human WRAP53 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BUR4
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies (PubMed:29695869, PubMed:29804836). Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC) (PubMed:19285445, PubMed:20351177, PubMed:29695869, PubMed:29804836). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes (PubMed:19179534, PubMed:20351177, PubMed:26170453, PubMed:29695869). In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex (PubMed:27525486, PubMed:29804836). Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity (PubMed:29804836). In addition, also controls telomerase holoenzyme complex localization to Cajal body (PubMed:22547674). During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity (PubMed:29804836). In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies (PubMed:19285445, PubMed:21072240). Also plays a role in DNA repair: phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks (PubMed:25512560, PubMed:27715493). Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ) (PubMed:25512560, PubMed:27715493).
Alternative names
TCAB1, WDR79, WRAP53, Telomerase Cajal body protein 1, WD repeat-containing protein 79, WD40 repeat-containing protein antisense to TP53 gene, WRAP53beta
Recommended products
Rabbit Polyclonal WDR79 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WRAP53 aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human WRAP53 aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BUR4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WDR79 also known as WRAP53 is a protein that assists in the assembly and maintenance of telomerase a ribonucleoprotein complex. It has a mass of approximately 80 kDa. The protein is mainly expressed in the nucleus of cells where it also interacts with components of Cajal bodies. Through these interactions WDR79 aids in the localization and stability of telomerase RNA (TERC) which is critical for telomerase activity. Additionally WDR79 interacts with p53 and acts as a cofactor for some spliceosomal small nuclear ribonucleoproteins (snRNPs).
- Biological function summary
WDR79 has a significant role in RNA processing and telomere maintenance. It is part of various ribonucleoprotein complexes where it serves as a scaffold facilitating RNA-protein interactions. WDR79 plays an essential role in the biogenesis of small Cajal body-specific RNAs (scaRNAs) which modify small nuclear RNAs (snRNAs). These modifications are necessary for proper splicing and maturation of pre-mRNA. Besides WDR79 is involved in the non-homologous end-joining (NHEJ) repair of double-strand breaks in DNA a critical cellular response to DNA damage.
- Pathways
WDR79 is centrally involved in telomere maintenance and RNA splicing pathways. In telomere maintenance it operates closely with the TERT and TERC components of the telomerase complex ensuring proper telomere elongation and genomic stability. In RNA splicing WDR79 interacts with proteins such as coilin which is integral to the organization and function of Cajal bodies where snRNP biogenesis occurs. The role of WDR79 in these pathways highlights its importance in regulating fundamental cellular processes.
- Associated diseases and disorders
Alterations in WDR79 function are linked to cancer and dyskeratosis congenita. Mutations and deregulation of this protein disturb telomere maintenance and cause genomic instability conditions that often lead to cancer development. WDR79 has connections to proteins such as TERT in these pathways which when dysregulated promote oncogenesis. In dyskeratosis congenita impaired telomerase function due to defective WDR79 interactions leads to stem cell failure and tissue degeneration underlining the protein’s importance in tissue maintenance and cellular longevity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-WDR79 antibody (ab224444)
All lanes: Western blot - Anti-WDR79 antibody (ab224444) at 0.4 µg/mL
Lane 1: Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2: WDR79 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)
Immunohistochemical analysis of human Fallopian tube tissue labeling WDR79 in the nucleus of glandular cells with ab224444 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-WDR79 antibody (ab224444)
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling WDR79 (green) in nuclear bodies with ab224444 at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)
Immunohistochemical analysis of human kidney tissue labeling WDR79 in the nucleus of cells in tubules with ab224444 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)
Immunohistochemical analysis of human skin tissue labeling WDR79 in the nucleus of squamous epithelial cells with ab224444 at 1/20 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (ab224444)
Immunohistochemical analysis of human liver tissue labeling WDR79 with ab224444 at 1/20 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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