AB99376

Anti-WDR79 antibody

Name: Anti-WDR79 antibody (ab99376) | Abcam
Brand: Abcam Limited
SKU: AB99376
Price: 525 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal WDR79 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human WRAP53 aa 1-50.

View Alternative Names

TCAB1, WDR79, WRAP53, Telomerase Cajal body protein 1, WD repeat-containing protein 79, WD40 repeat-containing protein antisense to TP53 gene, WRAP53beta

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (AB99376)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (AB99376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling WDR79 with ab99376 at 1/200 (1µg/ml). Detection : DAB.

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Immunoprecipitation - Anti-WDR79 antibody (AB99376)

Lane 1 : Detection of Human WDR79 by Immunoprecipitation from HeLa cells(1 mg for IP, 20% of IP loaded), using 3μg/mg lysate . Lane 2 : Control IgG

All lanes:

Immunoprecipitation - Anti-WDR79 antibody (ab99376)

Predicted band size: 59 kDa

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Unknown

Western blot - Anti-WDR79 antibody (AB99376)

All lanes:

Western blot - Anti-WDR79 antibody (ab99376) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 59 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human WRAP53 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BUR4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR79 also known as WRAP53 is a protein that assists in the assembly and maintenance of telomerase a ribonucleoprotein complex. It has a mass of approximately 80 kDa. The protein is mainly expressed in the nucleus of cells where it also interacts with components of Cajal bodies. Through these interactions WDR79 aids in the localization and stability of telomerase RNA (TERC) which is critical for telomerase activity. Additionally WDR79 interacts with p53 and acts as a cofactor for some spliceosomal small nuclear ribonucleoproteins (snRNPs).

Biological function summary

WDR79 has a significant role in RNA processing and telomere maintenance. It is part of various ribonucleoprotein complexes where it serves as a scaffold facilitating RNA-protein interactions. WDR79 plays an essential role in the biogenesis of small Cajal body-specific RNAs (scaRNAs) which modify small nuclear RNAs (snRNAs). These modifications are necessary for proper splicing and maturation of pre-mRNA. Besides WDR79 is involved in the non-homologous end-joining (NHEJ) repair of double-strand breaks in DNA a critical cellular response to DNA damage.

Pathways

WDR79 is centrally involved in telomere maintenance and RNA splicing pathways. In telomere maintenance it operates closely with the TERT and TERC components of the telomerase complex ensuring proper telomere elongation and genomic stability. In RNA splicing WDR79 interacts with proteins such as coilin which is integral to the organization and function of Cajal bodies where snRNP biogenesis occurs. The role of WDR79 in these pathways highlights its importance in regulating fundamental cellular processes.

Alterations in WDR79 function are linked to cancer and dyskeratosis congenita. Mutations and deregulation of this protein disturb telomere maintenance and cause genomic instability conditions that often lead to cancer development. WDR79 has connections to proteins such as TERT in these pathways which when dysregulated promote oncogenesis. In dyskeratosis congenita impaired telomerase function due to defective WDR79 interactions leads to stem cell failure and tissue degeneration underlining the protein’s importance in tissue maintenance and cellular longevity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA chaperone that plays a key role in telomere maintenance and RNA localization to Cajal bodies (PubMed : 29695869, PubMed : 29804836). Specifically recognizes and binds the Cajal body box (CAB box) present in both small Cajal body RNAs (scaRNAs) and telomerase RNA template component (TERC) (PubMed : 19285445, PubMed : 20351177, PubMed : 29695869, PubMed : 29804836). Essential component of the telomerase holoenzyme complex, a ribonucleoprotein complex essential for the replication of chromosome termini that elongates telomeres in most eukaryotes (PubMed : 19179534, PubMed : 20351177, PubMed : 26170453, PubMed : 29695869). In the telomerase holoenzyme complex, required to stimulate the catalytic activity of the complex (PubMed : 27525486, PubMed : 29804836). Acts by specifically binding the CAB box of the TERC RNA and controlling the folding of the CR4/CR5 region of the TERC RNA, a critical step for telomerase activity (PubMed : 29804836). In addition, also controls telomerase holoenzyme complex localization to Cajal body (PubMed : 22547674). During S phase, required for delivery of TERC to telomeres during S phase and for telomerase activity (PubMed : 29804836). In addition to its role in telomere maintenance, also required for Cajal body formation, probably by mediating localization of scaRNAs to Cajal bodies (PubMed : 19285445, PubMed : 21072240). Also plays a role in DNA repair : phosphorylated by ATM in response to DNA damage and relocalizes to sites of DNA double-strand breaks to promote the repair of DNA double-strand breaks (PubMed : 25512560, PubMed : 27715493). Acts by recruiting the ubiquitin ligase RNF8 to DNA breaks and promote both homologous recombination (HR) and non-homologous end joining (NHEJ) (PubMed : 25512560, PubMed : 27715493).

See full target information WRAP53

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Gynecologic and obstetric investigation 90:30-41 PubMed38657573

2024

CCT6A Regulates Cervical Cancer Cell Glycolysis and Proliferation under Hypoxic Conditions via the Telomerase Cajal Body Protein 1/Telomerase Reverse Tranase.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Hongli Wang

Journal of cell science 129:1566-79 PubMed26906424

2016

Telomerase activates transcription of cyclin D1 gene through an interaction with NOL1.

Applications

Unspecified application

Species

Unspecified reactive species

Juyeong Hong,Ji Hoon Lee,In Kwon Chung

Nucleic acids research 43:8435-51 PubMed26286192

2015

Involvement of SRSF11 in cell cycle-specific recruitment of telomerase to telomeres at nuclear speckles.

Applications

IP, ICC/IF

Species

Human, Human

Ji Hoon Lee,Sun Ah Jeong,Prabhat Khadka,Juyeong Hong,In Kwon Chung

Oncology letters 8:1009-1016 PubMed25120649

2014

Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to telomeres and induces G cell cycle arrest in A549 cells.

Applications

Species

Human

Ping Yuan,Zhitian Wang,Wang Lv,Hui Pan,Yunhai Yang,Xiaoshuai Yuan,Jian Hu

Genes & development 26:1897-910 PubMed22892240

2012

Human intron-encoded Alu RNAs are processed and packaged into Wdr79-associated nucleoplasmic box H/ACA RNPs.

Applications

Unspecified application

Species

Unspecified reactive species

Beáta E Jády,Amandine Ketele,Tamás Kiss

View all publications

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Product promise

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Anti-WDR79 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR79 antibody (AB99376)

Immunoprecipitation - Anti-WDR79 antibody (AB99376)

Western blot - Anti-WDR79 antibody (AB99376)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise