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AB249799

Anti-WDTC1 antibody [EPR12504] - BSA and Azide free

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Rabbit Recombinant Monoclonal WDTC1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

KIAA1037, WDTC1, WD and tetratricopeptide repeats protein 1

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)

This data was developed using ab174294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling WDTC1 with ab174294 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)

This data was developed using ab174294, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling WDTC1 with ab174294 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)

This data was developed using ab174294, the same antibody clone in a different buffer formulation.

ab174294 staining WDTC1 in HepG2 (Human hepatocellular carcinoma epithelial cell) cells by Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized n 0.1% TritonX-100. Samples were incubated with primary antibody at 1/50 dilution (1.6 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1/1000 dilution (2 μg/ml. An AlexaFluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker, ab195889 was used as a counterstain antibody (1/200, 2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining on HepG2 cell line.

Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)
  • IP

Supplier Data

Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)

This data was developed using ab174294, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab174294 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-WDTC1 antibody [EPR12504] (<a href='/en-us/products/primary-antibodies/wdtc1-antibody-epr12504-ab174294'>ab174294</a>)

Predicted band size: 76 kDa

false

Western blot - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)
  • WB

Supplier Data

Western blot - Anti-WDTC1 antibody [EPR12504] - BSA and Azide free (AB249799)

This data was developed using ab174294, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-WDTC1 antibody [EPR12504] (<a href='/en-us/products/primary-antibodies/wdtc1-antibody-epr12504-ab174294'>ab174294</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12504

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab249799 is the carrier-free version of ab174294.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDTC1 also known as Adipose is a protein that plays a role in various cellular processes. It is encoded by the WDTC1 gene and has a molecular weight of approximately 67 kDa. WDTC1 displays expression across many tissues with notable presence in adipose tissue and skeletal muscle. The protein contains WD40 repeats and a TBC (Tre-2/Bub2/Cdc16) domain which assist in protein-protein interactions and cellular trafficking.
Biological function summary

WDTC1 influences energy regulation and fat metabolism. It forms part of a protein complex that includes CUL4-DDB1 contributing to the ubiquitin-proteasome system. This activity regulates degradation of selective proteins ultimately controlling fat storage and mobilization. WDTC1 affects adipogenesis by modulating transcription factors and is involved in lipid metabolism through interactions with other cellular proteins.

Pathways

WDTC1 is actively involved in the regulation of metabolic pathways. It engages with the insulin signaling pathway and the PPARγ (peroxisome proliferator-activated receptor gamma) pathway both essential for energy homeostasis and fat cell differentiation. WDTC1 interacts with insulin receptor substrate proteins and partners with other ligase components to mediate the effects of these pathways on metabolism.

WDTC1 connects to obesity and type 2 diabetes. Variations in WDTC1 expression and function can disrupt normal fat metabolism contributing to these metabolic disorders. It associates with the hormone leptin a critical player in maintaining body weight linking WDTC1 to adipose tissue dysfunction. Understanding the role of WDTC1 may offer insight into therapeutic targets for metabolic disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
See full target information WDTC1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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