Rabbit Recombinant Monoclonal WEE1 antibody. Suitable for WB and reacts with Schizosaccharomyces pombe samples. Cited in 2 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | |
---|---|
Schizosaccharomyces pombe | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Schizosaccharomyces pombe | Dilution info 1/1000 | Notes - |
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Protein kinase that acts both on serines and on tyrosines. It acts as a dosage-dependent negative regulator of entry into mitosis (G2 to M transition). Phosphorylates and inhibits cdc2.
Mitosis inhibitor protein kinase wee1, P107 protein kinase homolog, wee1, SPCC18B5.03
Rabbit Recombinant Monoclonal WEE1 antibody. Suitable for WB and reacts with Schizosaccharomyces pombe samples. Cited in 2 publications.
Mitosis inhibitor protein kinase wee1, P107 protein kinase homolog, wee1, SPCC18B5.03
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR20899
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Wee1 also known as Wee1-like protein kinase or WEE1 serves as an important regulator of cell cycle progression. Wee1 is a protein kinase with a molecular mass of approximately 96 kDa involved in the regulation of the cell cycle by inhibiting the entry into mitosis through phosphorylation of cyclin-dependent kinase 1 (CDK1). Expression of Wee1 occurs throughout various tissues but it is especially important in those that require tight control over cell division like the brain and reproductive organs. By suppressing premature mitosis Wee1 ensures cells have adequate time for DNA repair and completion of critical processes before cell division.
The function of Wee1 extends to its role in maintaining genomic stability. Wee1 operates as part of a regulatory complex and its inhibition results in defective cell cycle arrest potentially leading to DNA damage. The kinase acts to prevent transitions from the G2 to M phase of the cell cycle ensuring cells repair damaged DNA before division. In the context of DNA replication stress Wee1 cooperates with other regulators such as Chk1 to mediate cell cycle arrest therefore safeguarding genomic integrity.
The role of Wee1 manifests significantly within the DNA damage checkpoint pathway and the cell cycle control pathway. In the DNA damage checkpoint pathway Wee1 collaborates with other cell cycle regulators such as ATR and Chk1 to control the cell cycle in response to DNA damages. Wee1's influence on the cell cycle pathway also intersects with CDK1 and Cyclin B where Wee1 modulates the activity of these proteins to control cell cycle transitions. This regulatory action allows cells to coordinate DNA repair and replication with cell division events.
Wee1's regulatory functions relate closely to cancer and neurological disorders. Overexpression or mutation of Wee1 is associated with various cancers including gliomas and breast cancer where it influences cell proliferation by controlling the activity of CDK1. Wee1's relationship with cancer extends to its interactions with p53 and Chk1 proteins both of which are critical in cancer biology. Additionally anomalies in Wee1 expression or function also associate with certain neurological disorders where it may alter cell cycle dynamics and influence neural cell fate under stress conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Blocking and dilution buffer: 5% NFDM/TBST.
ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and Anti-Tubulin antibody [EPR13796] - Loading Control ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.
All lanes: Western blot - Anti-Wee1 antibody [EPR20899] (ab233540) at 1/1000 dilution
Lane 1: Wild-type Schizosaccharomyces pombe whole cell lysate at 10 µg
Lane 2: Wee1 knockout Schizosaccharomyces pombe whole cell lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 72 kDa
Observed band size: 110 kDa
Exposure time: 70s
Blocking and dilution buffer: 5% NFDM/TBST.
ab233540 was shown to specifically react with Wee1 in wild-type fission yeast cells as signal was lost in Wee1 knockout cells. Wild-type and Wee1 knockout samples were subjected to SDS-PAGE. ab233540 and Anti-Tubulin antibody [EPR13796] - Loading Control ab210797 (Rabbit anti-Tubulin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Wee1 knockout and WT fission yeast whole cell lysates were kindly provided by Dr Quanwen Jin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233540).
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