Skip to main content

Rabbit Recombinant Monoclonal WEE1 phospho S642 antibody. Suitable for Dot, IHC-P, WB and reacts with Human, Rat, Mouse samples.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (AB307515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (AB307515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (AB307515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (AB307515), expandable thumbnail
  • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (AB307515), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Expected
Not recommended
Not recommended
Not recommended
Tested
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

    Alternative names

    Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1

    Recommended products

    1. Loading...
    2. Loading...
    3. Loading...
    4. Loading...

    Rabbit Recombinant Monoclonal WEE1 phospho S642 antibody. Suitable for Dot, IHC-P, WB and reacts with Human, Rat, Mouse samples.

    Alternative names

    Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogens
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR27036-80

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    8 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Wee1 (phospho S642) with ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on spermatogonia of rat testis without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab307515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Wee1 (phospho S642) with ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on spermatogonia of mouse testis without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab307515 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Wee1 (phospho S642) with ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on spermatogonia of human testis without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab307515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      This data was developed using ab307515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Wee1 (phospho S642) with ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human breast carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab307515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a high sensitivity ECL substrate.

      Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
      Exposure time: 180 seconds

      All lanes: Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515) at 1/1000 dilution

      Lane 1: Mouse testis tissue lysate 20 µg (untreated membrane)

      Lane 2: Mouse testis tissue lysate 20 µg (phosphatase treated membrane)

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Observed band size: 95 kDa

      Exposure time: 180s

    • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Exposure time: Lanes 1-4: 15 seconds, Lanes 5-6: 180 seconds.

      All lanes: Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515) at 1/1000 dilution

      Lane 1: Untreated A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 20 μg (untreated membrane)

      Lane 2: A431 treated with100nM Calycin A for 30min whole cell lysate 20 μg (untreated membrane)

      Lane 3: A431 treated with100nM Calycin A for 30min whole cell lysate 20 μg (phosphatase treated membrane)

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 95 kDa

      Exposure time: 180s

    • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Exposure time: 59 seconds

      All lanes: Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515) at 1/1000 dilution

      Lane 1: Untreated 293T cells transfected with a human Wee1 expression vector containi a His-tag whole cell lysate 20 μg

      Lane 2: 293T cells transfected with a human Wee1 expression vector containi a His-tag, treated with 100nM Calyculin A for 30min whole cell lysate 20 μg

      Lane 3: Untreated 293T cells transfected with a human Wee1(S642A) expression vector containi a His-tag whole cell lysate 20 μg

      Lane 4: 293T cells transfected with a human Wee1(S642A) expression vector containi a His-tag, treated with 100nM Calyculin A for 30min whole cell lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 45 kDa, 95 kDa

      Exposure time: 59s

    • Dot Blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515), expandable thumbnail

      Dot Blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (ab307515)

      Dot blot analysis of Wee1 (phospho S642) using ab307515 at 1:1000 (0.488 ug/ml) followed by a Goat Anti-RABbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

      Exposure time: 3 minutes
      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com