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Rabbit Recombinant Monoclonal WEE1 phospho S642 antibody. Carrier free. Suitable for Dot, IHC-P, WB and reacts with Human, Rat, Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (AB307516), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (AB307516), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (AB307516), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (AB307516), expandable thumbnail
  • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (AB307516), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotIPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Expected
Not recommended
Not recommended
Not recommended
Tested
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Tested
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human, Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

    Alternative names

    Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1

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    Rabbit Recombinant Monoclonal WEE1 phospho S642 antibody. Carrier free. Suitable for Dot, IHC-P, WB and reacts with Human, Rat, Mouse samples.

    Alternative names

    Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogens
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR27036-80

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    Wee1 also known as Wee1-like protein kinase or WEE1 serves as an important regulator of cell cycle progression. Wee1 is a protein kinase with a molecular mass of approximately 96 kDa involved in the regulation of the cell cycle by inhibiting the entry into mitosis through phosphorylation of cyclin-dependent kinase 1 (CDK1). Expression of Wee1 occurs throughout various tissues but it is especially important in those that require tight control over cell division like the brain and reproductive organs. By suppressing premature mitosis Wee1 ensures cells have adequate time for DNA repair and completion of critical processes before cell division.

    Biological function summary

    The function of Wee1 extends to its role in maintaining genomic stability. Wee1 operates as part of a regulatory complex and its inhibition results in defective cell cycle arrest potentially leading to DNA damage. The kinase acts to prevent transitions from the G2 to M phase of the cell cycle ensuring cells repair damaged DNA before division. In the context of DNA replication stress Wee1 cooperates with other regulators such as Chk1 to mediate cell cycle arrest therefore safeguarding genomic integrity.

    Pathways

    The role of Wee1 manifests significantly within the DNA damage checkpoint pathway and the cell cycle control pathway. In the DNA damage checkpoint pathway Wee1 collaborates with other cell cycle regulators such as ATR and Chk1 to control the cell cycle in response to DNA damages. Wee1's influence on the cell cycle pathway also intersects with CDK1 and Cyclin B where Wee1 modulates the activity of these proteins to control cell cycle transitions. This regulatory action allows cells to coordinate DNA repair and replication with cell division events.

    Associated diseases and disorders

    Wee1's regulatory functions relate closely to cancer and neurological disorders. Overexpression or mutation of Wee1 is associated with various cancers including gliomas and breast cancer where it influences cell proliferation by controlling the activity of CDK1. Wee1's relationship with cancer extends to its interactions with p53 and Chk1 proteins both of which are critical in cancer biology. Additionally anomalies in Wee1 expression or function also associate with certain neurological disorders where it may alter cell cycle dynamics and influence neural cell fate under stress conditions.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    8 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Wee1 (phospho S642) with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on spermatogonia of rat testis without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Wee1 (phospho S642) with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on spermatogonia of mouse testis without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Wee1 (phospho S642) with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on spermatogonia of human testis without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Wee1 (phospho S642) with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 at 1/2000 (0.244 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human breast carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

    • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using 307515, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration: This blot was developed using a high sensitivity ECL substrate.

      Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
      180 seconds
      Exposure time:

      All lanes: Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515) at 1/1000 dilution

      Lane 1: Mouse testis tissue lysate 20 µg (untreated membrane)

      Lane 2: Mouse testis tissue lysate 20 µg (phosphatase treated membrane)

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Developed using the ECL technique.

      Observed band size: 95 kDa

      Exposure time: 180s

      Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a high sensitivity ECL substrate.

      Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
      Exposure time: 180 seconds

    • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using 307515, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration:
      Lanes 1-4: 15 seconds, Lanes 5-6: 180 seconds.
      Exposure time:

      All lanes: Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515) at 1/1000 dilution

      Lane 1: Untreated A431 (human epidermoid carcinoma epithelial cell) whole cell lysate 20 μg (untreated membrane)

      Lane 2: A431 treated with100nM Calycin A for 30min whole cell lysate 20 μg (untreated membrane)

      Lane 3: A431 treated with100nM Calycin A for 30min whole cell lysate 20 μg (phosphatase treated membrane)

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

      Observed band size: 95 kDa

      Exposure time: 180s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Exposure time: Lanes 1-4: 15 seconds, Lanes 5-6: 180 seconds.

    • Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using 307515, the same antibody clone in a different buffer formulation.
      Blocking and diluting buffer and concentration:
      59 seconds
      Exposure time:

      All lanes: Western blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] (Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515) at 1/1000 dilution

      Lane 1: Untreated 293T cells transfected with a human Wee1 expression vector containi a His-tag whole cell lysate 20 μg

      Lane 2: 293T cells transfected with a human Wee1 expression vector containi a His-tag, treated with 100nM Calyculin A for 30min whole cell lysate 20 μg

      Lane 3: Untreated 293T cells transfected with a human Wee1(S642A) expression vector containi a His-tag whole cell lysate 20 μg

      Lane 4: 293T cells transfected with a human Wee1(S642A) expression vector containi a His-tag, treated with 100nM Calyculin A for 30min whole cell lysate 20 μg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 45 kDa, 95 kDa

      Exposure time: 59s

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      Exposure time: 59 seconds

    • Dot Blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516), expandable thumbnail

      Dot Blot - Anti-Wee1 (phospho S642) antibody [EPR27036-80] - BSA and Azide free (ab307516)

      This data was developed using Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515, the same antibody clone in a different buffer formulation.
      Dot blot analysis of Wee1 (phospho S642) using Anti-Wee1 (phospho S642) antibody [EPR27036-80] ab307515 at 1:1000 (0.488 ug/ml) followed by a Goat Anti-RABbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

      Exposure time: 3 minutes
      Blocking and diluting buffer and concentration: 5% NFDM/TBST

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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